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Series GSE98242 Query DataSets for GSE98242
Status Public on Feb 15, 2018
Title The AP-1 Transcription Factor JunB Is Required for Th17 Cell Differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Interleukin (IL)-17-producing T helper (Th17) cells are crucial for host defense against extracellular microbes and pathogenesis of autoimmune diseases. Here we show that the AP-1 transcription factor JunB is required for Th17 cell development. Junb-deficient CD4+ T cells are able to develop in vitro into various helper T subsets except Th17. The RNA-seq transcriptome analysis reveals that JunB is crucial for the Th17-specific gene expression program. Junb-deficient mice are completely resistant to experimental autoimmune encephalomyelitis, a Th17-mediated inflammatory disease, and naive T helper cells from such mice fail to differentiate into Th17 cells. JunB appears to activate Th17 signature genes by forming a heterodimer with BATF, another AP-1 factor essential for Th17 differentiation. The mechanism whereby JunB controls Th17 cell development likely involves activation of the genes for the Th17 lineage-specifying orphan receptors RORt and ROR and reduced expression of Foxp3, a transcription factor known to antagonize RORt function.
Overall design Naïve T cell, Th0 and Th17 mRNA profiles of wild type (WT) and Junb-/- mice were generated by deep sequencing, using Illumina HiSeq2500
Contributor(s) Yamazaki S, Tanaka Y, Araki H, Kohda A, Sanematsu F, Arasaki T, Duan X, Miura F, Ito T, Fukui Y, Endo S, Sumimoto H
Citation(s) 29234109
Submission date Apr 26, 2017
Last update date Jul 25, 2021
Contact name Hiromitsu Araki
Organization name Kyushu University
Street address 744 Motooka Nishi-Ku
City Fukuoka
ZIP/Postal code 819-0395
Country Japan
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM2589764 WT, naïve T cell
GSM2589765 WT, Th0 cell
GSM2589766 WT, Th17 cell, replicate 1
BioProject PRJNA384377
SRA SRP105275

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Supplementary file Size Download File type/resource
GSE98242_RAW.tar 15.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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