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Series GSE98427 Query DataSets for GSE98427
Status Public on Feb 22, 2018
Title Epigenetic activation during Th17 cell differentiation is impaired after TRIM28 deletion
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Cell fate decision is mediated by epigenetic mechanisms. We have analyzed naive T cell differentiation into Th17 cells, which is regulated by environmental cytokines and their downstream transcription factors. RORγt is a lineage-specific master transcription factor for Th17 cells. Although epigenetic mechanisms have been implicated in Th17 cell differentiation, how transcription factors interact to activate epigenetic program is unclear. Here we show that tripartite motif containing 28 (TRIM28) expression in Th17 cells is required for cytokine production and autoimmune diseases. Genome-wide occupancy analysis reveals that TRIM28 bound regions contain many super-enhancers, which are impaired after TRIM28 or STAT3 but not RORγt deletion. Importantly, TRIM28 exists in a complex with STAT3 and RORγt; TRIM28 recruitment to the Il17 gene requires STAT3, and further promotes RORγt recruitment. TRIM28 thus is a key player in the epigenetic activation during T cell differentiation.
Overall design 8 samples in total: 2 RNA-seq samples; 6 ChIP-seq samples, including 3 control samples(input)
Contributor(s) Jiang Y, Lu H, Liu Y, Sun S, Jin W, Wang X, Dong C
Citation(s) 29651155
Submission date May 02, 2017
Last update date Jul 25, 2021
Contact name Huiping Lu
Phone 010-62797352
Organization name Tsinghua university
Street address Tsinghua university
City Beijing
ZIP/Postal code 100084
Country China
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM2595276 WT Th17
GSM2595277 TRIM28KO Th17
GSM2595278 WT p300
BioProject PRJNA385079
SRA SRP106014

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE98427_RAW.tar 480.2 Mb (http)(custom) TAR (of TDF, TXT, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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