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Series GSE98502 Query DataSets for GSE98502
Status Public on Jun 01, 2018
Title Gene expression profiling coupled with the Connectivity Map database-mining reveals potential therapeutic drugs for Hirschsprung disease
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Hirschsprung disease (HD) is a congenital intestinal anomaly, which derives from a failure to form enteric ganglia in the lower bowel. Surgery is a major therapeutic strategy and neural stem cells transplantation is believed as a promising approach. However, we still face many challenges. To better characterize the pathology of HD, we used Agilent microarrays to analyze the gene expression profiles of surgically resected bowel tissue from 8 HD patients and 8 controls. A total of 253 genes were differentially expressed between the two groups using SAM software with threshold set at fold-change >2.0 and q-value <0.05. Gene Ontology analysis demonstrated that genes related to regulation of neuron development were significantly enriched. Differentially expressed genes (DEGs) involved in neuron development were further validated by quantitative real-time PCR (qRT-PCR) and immunohistochemistry. Moreover, in an attempt to seek potential drugs for HD, we correlated the observed gene expression patterns in HD to those of small molecule compounds via the Connectivity Map (cMap) database. Several compounds were screened that could rescue the dysregulated molecular signature in HD, proposing a testable hypothesis about possible therapeutic agents as a novel remedy for HD. In conclusion, our work may enhance our understanding the molecular changes in HD. More importantly, this study opens new fields of investigation into pharmacological intervention for HD.
 
Overall design A total of 8 children diagnosed with congenital megacolon, 7 boys and 1 girl, age range from 1 to 6 months, were recruited in our series. The dual-color approach was employed in this experiment. Individual diseased samples and their paired adjacent normal tissues were all labeled with Cy3 and hybridized with a common reference consisted of 8 normal controls labeled with Cy5.
 
Contributor(s) Xiao S, Zhu X, Deng H, Zhou W, Yang W, Yuan L, Zhang J, Zhang L, Xia H
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 03, 2017
Last update date Jul 25, 2021
Contact name Liang Zhang
Organization name Guangdong Women and Children Hospital
Department Translational Medicine Research Center
Street address Xingnan Road 521
City Guangzhou
State/province Guangdong
ZIP/Postal code 510010
Country China
 
Platforms (1)
GPL22361 Agilent-071342 LncRNA [Feature number version]
Samples (16)
GSM2597481 CK-1
GSM2597482 CK-2
GSM2597483 CK-3
Relations
BioProject PRJNA385302

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98502_RAW.tar 85.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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