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Series GSE99529 Query DataSets for GSE99529
Status Public on Jul 12, 2017
Title Identification of LEC1 binding sites in Arabidopsis seedlings
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary LEAFY COTYLEDON1 (LEC1), an atypical subunit of the NF-Y CCAAT binding transcription factor, is a central regulator that controls many aspects of seed development including the maturation phase during which seeds accumulate storage macromolecules and embryos acquire the ability to withstand desiccation. To define the gene network and developmental processes controlled by LEC1, genes regulated directly by and downstream of LEC1 were identified. In this part of the study, we identified the genes bound by LEC1 by overexpressing a FLAG-tagged inducible form of LEC1 in Arabidopsis seedlings and inducing LEC1 activity for a short (4 hours) or long (8 days) period of time, to identify the genes bound EARLY and LATE, respectively.
Overall design LEC1 activity was induced in Arabidopsis 35S:FLAG-LEC1-GR plants, either by treating seedlings for 4h, or germinating seeds and growing seedlings for 8 days in the presence of dexamethasone (DEX). Anti-FLAG antibodies (Sigma F1804) were used to immunoprecipitate LEC1-bound DNA fragments and compared to total fragmented chromatin from the same biological materials. Two biological replicates per conditions were analyzed.
Contributor(s) Pelletier J, Kwong RW, Bui AQ, Le BH, Golberg RB, Harada JJ
Citation(s) 28739919
Submission date May 31, 2017
Last update date Jul 25, 2021
Contact name John Harada
Organization name University of California
Department Plant Biology
Lab Harada lab
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platforms (1)
GPL10977 [At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array
Samples (8)
GSM2645487 FL1GR_4hD_FLAG3
GSM2645488 FL1GR_4hD_FLAG4
GSM2645489 FL1GR_4hD_INP3
BioProject PRJNA388705

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99529_FL1GR_4hD_peaks.bed.gz 24.6 Kb (ftp)(http) BED
GSE99529_FL1GR_8dD_peaks.bed.gz 37.7 Kb (ftp)(http) BED
GSE99529_RAW.tar 418.1 Mb (http)(custom) TAR (of BAR, CEL)
Processed data provided as supplementary file
Processed data are available on Series record

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