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Series GSE99873 Query DataSets for GSE99873
Status Public on Aug 25, 2017
Title Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies II
Platform organisms Quercus alba; Gossypium hirsutum; Rhodotorula; Trypanosoma cruzi; Pandalus borealis; Oncorhynchus nerka; Gallus gallus; Homo sapiens; Sus scrofa domesticus; Bos taurus; Ovis aries; Oryctolagus cuniculus; Mus musculus; Human immunodeficiency virus 1; Ceratonia siliqua; Megathura crenulata; Bothrops moojeni; Frangula alnus; Senegalia senegal; Pachymenia carnosa; Dermestes lardarius
Sample organisms Homo sapiens; Mus musculus
Experiment type Protein profiling by protein array
Summary Purpose: This study uses a high-throughput glycan microarray to evaluate the immunological evolution of antibodies to the glyco-antigen GD2. The goal is to determine germline and affinity mature antibody specificity and affinities/
Results: Affinity mature anti-GD2 antibodies 3F8 and ch14.18 had high affinity and were highly specific for the target GD2. Germline antibodies were also hihgly specific and had surprisingly high affinity.
Conclusion: Antibodies to GD2 evolved from highly specific germlines. Highly specific germlines may be critical in evading autoimmunity issues.
 
Overall design Germline variable regions of 3F8 and ch14.18 were determined. Germline and affinity mature antibody DNA were synthesized and transfected into HEK293 cell lines. Secreted antibodies were purified from supernatant using affinity chromatography. Antibodies were evaluated on multiple assay platforms, including glycan microarrays, protein microarrays, and cell binding assays.
 
Contributor(s) Sterner E, Peach ML, Nicklaus MC, Gildersleeve JC
Citation(s) 28813678
Submission date Jun 09, 2017
Last update date Jan 24, 2018
Contact name Jeff Gildersleeve
Organization name National Cancer Institute
Street address 376 Boyles St.
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platforms (1)
GPL23566 PROSTVAC a96 ver. 2
Samples (14)
GSM2656563 Affinity mature ch14.18 -- Anti-GD2 Antibody ch14.18_1
GSM2656564 Affinity mature ch14.18 -- Anti-GD2 Antibody ch14.18_2
GSM2656565 Affinity mature ch14.18 -- Anti-GD2 Antibody ch14.18_3
This SubSeries is part of SuperSeries:
GSE100438 Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies
Relations
BioProject PRJNA391734

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99873_2015-07-29_217_A503_Batch_A_Slide_7.gpr.gz 1.0 Mb (ftp)(http) GPR
GSE99873_2015-08-10_217_A503_Batch_A_Slide_5.gpr.gz 1.0 Mb (ftp)(http) GPR
GSE99873_2015-08-25_217_A503_Batch_A_Slide_2.gpr.gz 1.0 Mb (ftp)(http) GPR
GSE99873_README_glycan_arrays_text.txt 13.1 Kb (ftp)(http) TXT
Processed data included within Sample table

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