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| Status |
Public on Jun 13, 2019 |
| Title |
TNF-induced gene-expressions in Nfkb2-/- MEFs and its derivatives |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
As such, TNF triggers the canonical NF-?B pathway to induce a nuclear NF-?B activity composed of the RelA:p50 dimer in WT cells. Nfkb2 encodes p100, which regulates the activity of the RelB NF-?B heterodimers during immune cell-differentiation via the noncanonical pathway. Our biochemical analyses revealed that an absence of p100 instead repositions RelB under the control of the TNF-activated canonical pathway. Indeed, chronic TNF treatment of Nfkb2-/- cells activates the RelA:p50 dimer and an additional RelB:p50 dimer. On the other hand, TNF stimulation of Relb-/-Nfkb2-/- and Rela-/-Nfkb2-/- MEFs exclusively activated the RelA:p50 dimer and the RelB:p50 dimer, respectively. We treated this panel of knockout MEFs with TNF for 6h before being subjected to microarray mRNA analysis. We also included in our analysis WT MEFs and Rela-/-Relb-/-Rel-/- MEFs, which served as a negative control. Our analyses revealed overlapping and distinct gene-expression specificities of RelA:p50 and RelB:p50 dimers activated in mutant cells in response to TNF.
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| Overall design |
Total RNA isolated from biological replicate samples comprising of untreated and 6h TNF (10ng/ml) treated WT, Nfkb2-/-, Rela-/-Nfkb2-/-, Relb-/-Nfkb2-/-and Rela-/-Relb-/-Rel-/- MEFs were analyzed for global gene expression levels
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| Contributor(s) |
Basak S, Roy P, Chatterjee B, Sarkar UA |
| Citation missing |
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| |
| Submission date |
Jun 13, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Soumen Basak |
| Organization name |
National Institute of Immunology
|
| Department |
Infection and immunity
|
| Lab |
Systems Immunology Laboratory
|
| Street address |
Aruna Asaf Ali Marg
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA390927 |
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