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Sample GSM1005317 Query DataSets for GSM1005317
Status Public on Jul 31, 2013
Title 2-SD-L
Sample type RNA
Source name liver tissue
Organism Mus musculus
Characteristics tissue: Liver
age: one-year old
gender: male
strain: B6C57/J
diet: maintained on ad libitum AIN-93G SD until the end of their life
Treatment protocol Liver tissue from one-year old male B6C57/J mice maintained on ad libitum AIN-93G SD until the end of their life.
Extracted molecule total RNA
Extraction protocol RNA was extracted from liver tissue using 1.0mm glass beads (BioSpec Products, Inc.) in a Precellys 24 Tissue Homogenizer (Bertin Technologies) and Qiagen RNeasy Mini Kits according to manufacturer's specifications. Quality and quantity of the total RNA was checked with the Agilent 2100 bioanalyzer using RNA 6000 Nano chips.
Label Streptavidin-Cy3 bound to biotin-labeled cRNA.
Label protocol Standard Illumina protocol using Illumina TotalPrep RNA Amplification Kit (Ambion; Austin, TX, cat # IL1791) In short, 0.5g of total RNA was first converted into single-stranded cDNA with reverse transcriptase using an oligo-dT primer containing the T7 RNA polymerase promoter site and then copied to produce double-stranded cDNA molecules. The double stranded cDNA was cleaned and concentrated with the supplied columns and used in an overnight in-vitro transcription reaction where single-stranded RNA (cRNA) was generated and labeled by incorporation of biotin-16-UTP.
Hybridization protocol Standard Illumina protocol. In short, a total of 0.75ug of biotin-labeled cRNA was hybridized at 58 degrees C for 16 hours to Illumina's Sentrix MouseRef-8 v2 Expression BeadChips (Illumina, San Diego, CA). Each BeadChip has ~24,000 well-annotated RefSeq transcripts with approximately 30-fold redundancy. The arrays were washed, blocked and the biotin labeled cRNA was detected by staining with streptavidin-Cy3.
Scan protocol Arrays were scanned at a resolution of 0.8um using the Beadstation 500 X from Illumina.
Description supplementary file:GEO_Supplementary_file_AS_Liver 9-14-12
Liver tissue from one-year old male B6C57/J mice maintained on ad libitum AIN-93G SD until the end of their life.
Data processing Data was extracted using the Illumina GenomeStudio software(v1.6.0). Any spots at or below the background were filtered out using an Illumina detection p value of 0.02 and above. The natural log of all remaining scores were used to find the avg and std of each array and the z-score normalization was calculated and presented below. Z-score = (raw value - avg)/std. Complete data including detection scores will be included in the supplemental file.
Submission date Sep 17, 2012
Last update date Jun 22, 2020
Contact name Supriyo De
Organization name NIA-IRP, NIH
Department Laboratory of Genetics and Genomics
Lab Computational Biology & Genomics Core
Street address 251 Bayview Blvd
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
Platform ID GPL6885
Series (1)
GSE40936 Metformin mild supplementation improves health and survival of mice

Data table header descriptions
RAW_VALUE Raw intensity value from Illumina GenomeStudio software
VALUE Z transformation of the natural log of the raw intensity values

Data table
ILMN_1212612 192.4 -0.318094659
ILMN_1212632 239.7 -0.122725462
ILMN_1212636 2586.2 1.991342751
ILMN_1212637 3301.8 2.208457893
ILMN_1212645 8144.4 3.010924995
ILMN_1212648 249.6 -0.086754279
ILMN_1212653 193.5 -0.313027617
ILMN_1212672 477.3 0.489443851
ILMN_1212693 299.3 0.074640582
ILMN_1212702 10628.9 3.247565338
ILMN_1212703 308.9 0.102701144

Total number of rows: 25694

Table truncated, full table size 591 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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