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Sample GSM1012269 Query DataSets for GSM1012269
Status Public on Feb 06, 2013
Title Control morpholino, injected side, replicate 2
Sample type RNA
Source name control morpholino, injected side
Organism Xenopus laevis
Characteristics tissue: embryo
treatment: control morpholino
side: injected
Treatment protocol Morpholinos were injected in two neighbouring, animal or vegetal blastomeres on one side of the embryo, at half the dose (i.e. 40 ng total).
Growth protocol Xenopus laevis eggs were collected, fertilized in vitro and handled following standard procedures. Embryos were staged according to Nieuwkoop and Faber (1967).
Extracted molecule total RNA
Extraction protocol Total cellular RNA was isolated with TRIzol (Qiagen) and phenol/chloroform extraction. On-column RNA clean-up, including a DNase digestion step, was performed using the RNeasy-Mini-Kit (Qiagen).
Label biotin
Label protocol Standard Affymetrix protocol.
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description X. laevis embryos, control knockdown, injected side, replicate 2.
Data processing R/Bioconductor, gcrma normalization, default parameters.
Submission date Oct 01, 2012
Last update date Feb 06, 2013
Contact name Tobias Straub
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address GroƟhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL10756
Series (1)
GSE41256 Suv4-20h Histone Methyltransferases Promote Neuroectodermal Differentiation by Silencing the Pluripotency-Associated Oct-25 Gene

Data table header descriptions
VALUE GC-RMA expression value

Data table
AFFX-BioB-3_at 8.427851947
AFFX-BioB-5_at 8.151246738
AFFX-BioB-M_at 8.859979126
AFFX-BioC-3_at 10.29534264
AFFX-BioC-5_at 9.705677029
AFFX-BioDn-3_at 12.19176343
AFFX-BioDn-5_at 11.69099077
AFFX-CreX-3_at 14.17000266
AFFX-CreX-5_at 13.98483303
AFFX-DapX-3_at 11.90934298
AFFX-DapX-5_at 8.610275571
AFFX-DapX-M_at 10.58456316
AFFX-LysX-3_at 8.555186673
AFFX-LysX-5_at 6.767574685
AFFX-LysX-M_at 7.250362657
AFFX-PheX-3_at 8.365008515
AFFX-PheX-5_at 7.066527278
AFFX-PheX-M_at 7.927260212
AFFX-r2-Bs-dap-3_at 11.89838546
AFFX-r2-Bs-dap-5_at 9.102608333

Total number of rows: 32635

Table truncated, full table size 947 Kbytes.

Supplementary file Size Download File type/resource
GSM1012269_C_MO_80_INJ_Exp2.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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