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Sample GSM1012271 Query DataSets for GSM1012271
Status Public on Feb 06, 2013
Title Control morpholino, uninjected side, replicate 2
Sample type RNA
 
Source name control morpholino, uninjected side
Organism Xenopus laevis
Characteristics tissue: embryo
treatment: control morpholino
side: uninjected
Treatment protocol Morpholinos were injected in two neighbouring, animal or vegetal blastomeres on one side of the embryo, at half the dose (i.e. 40 ng total).
Growth protocol Xenopus laevis eggs were collected, fertilized in vitro and handled following standard procedures. Embryos were staged according to Nieuwkoop and Faber (1967).
Extracted molecule total RNA
Extraction protocol Total cellular RNA was isolated with TRIzol (Qiagen) and phenol/chloroform extraction. On-column RNA clean-up, including a DNase digestion step, was performed using the RNeasy-Mini-Kit (Qiagen).
Label biotin
Label protocol Standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description X. laevis embryos, control knockdown, uninjected side, replicate 2.
Data processing R/Bioconductor, gcrma normalization, default parameters.
 
Submission date Oct 01, 2012
Last update date Feb 06, 2013
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address GroƟhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL10756
Series (1)
GSE41256 Suv4-20h Histone Methyltransferases Promote Neuroectodermal Differentiation by Silencing the Pluripotency-Associated Oct-25 Gene

Data table header descriptions
ID_REF
VALUE GC-RMA expression value

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.700472422
AFFX-BioB-5_at 8.300742363
AFFX-BioB-M_at 9.031607156
AFFX-BioC-3_at 10.47313739
AFFX-BioC-5_at 9.863428785
AFFX-BioDn-3_at 12.34059298
AFFX-BioDn-5_at 11.86534612
AFFX-CreX-3_at 14.2979813
AFFX-CreX-5_at 14.10111357
AFFX-DapX-3_at 11.70911533
AFFX-DapX-5_at 8.625266469
AFFX-DapX-M_at 10.45831828
AFFX-LysX-3_at 8.442438678
AFFX-LysX-5_at 6.410859044
AFFX-LysX-M_at 6.962300925
AFFX-PheX-3_at 8.162556489
AFFX-PheX-5_at 6.804951395
AFFX-PheX-M_at 7.578918186
AFFX-r2-Bs-dap-3_at 11.7455335
AFFX-r2-Bs-dap-5_at 8.986390909

Total number of rows: 32635

Table truncated, full table size 947 Kbytes.




Supplementary file Size Download File type/resource
GSM1012271_C_MO_80_UNINJ_Exp2.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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