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Sample GSM1012272 Query DataSets for GSM1012272
Status Public on Feb 06, 2013
Title suv420 morpholino, injected side, replicate 1
Sample type RNA
 
Source name suv420 morpholino, injected side
Organism Xenopus laevis
Characteristics tissue: embryo
treatment: suv420 morpholino
side: injected
Treatment protocol Morpholinos were injected in two neighbouring, animal or vegetal blastomeres on one side of the embryo, at half the dose (i.e. 40 ng total).
Growth protocol Xenopus laevis eggs were collected, fertilized in vitro and handled following standard procedures. Embryos were staged according to Nieuwkoop and Faber (1967).
Extracted molecule total RNA
Extraction protocol Total cellular RNA was isolated with TRIzol (Qiagen) and phenol/chloroform extraction. On-column RNA clean-up, including a DNase digestion step, was performed using the RNeasy-Mini-Kit (Qiagen).
Label biotin
Label protocol Standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description X. laevis embryos, suv420 knockdown, injected side, replicate 1.
Data processing R/Bioconductor, gcrma normalization, default parameters.
 
Submission date Oct 01, 2012
Last update date Feb 06, 2013
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address GroƟhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL10756
Series (1)
GSE41256 Suv4-20h Histone Methyltransferases Promote Neuroectodermal Differentiation by Silencing the Pluripotency-Associated Oct-25 Gene

Data table header descriptions
ID_REF
VALUE GC-RMA expression value

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.706481917
AFFX-BioB-5_at 8.354418609
AFFX-BioB-M_at 9.20829306
AFFX-BioC-3_at 10.44588595
AFFX-BioC-5_at 9.945148504
AFFX-BioDn-3_at 12.43715413
AFFX-BioDn-5_at 11.94427304
AFFX-CreX-3_at 14.35824884
AFFX-CreX-5_at 14.18162884
AFFX-DapX-3_at 11.99221163
AFFX-DapX-5_at 8.897261903
AFFX-DapX-M_at 10.7285211
AFFX-LysX-3_at 8.772809357
AFFX-LysX-5_at 6.785801216
AFFX-LysX-M_at 7.341033979
AFFX-PheX-3_at 8.478648213
AFFX-PheX-5_at 7.159773758
AFFX-PheX-M_at 8.107073356
AFFX-r2-Bs-dap-3_at 11.97762304
AFFX-r2-Bs-dap-5_at 9.242764009

Total number of rows: 32635

Table truncated, full table size 947 Kbytes.




Supplementary file Size Download File type/resource
GSM1012272_S_MO_80_INJ_Exp1.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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