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Sample GSM1012273 Query DataSets for GSM1012273
Status Public on Feb 06, 2013
Title suv420 morpholino, injected side, replicate 2
Sample type RNA
Source name suv420 morpholino, injected side
Organism Xenopus laevis
Characteristics tissue: embryo
treatment: suv420 morpholino
side: injected
Treatment protocol Morpholinos were injected in two neighbouring, animal or vegetal blastomeres on one side of the embryo, at half the dose (i.e. 40 ng total).
Growth protocol Xenopus laevis eggs were collected, fertilized in vitro and handled following standard procedures. Embryos were staged according to Nieuwkoop and Faber (1967).
Extracted molecule total RNA
Extraction protocol Total cellular RNA was isolated with TRIzol (Qiagen) and phenol/chloroform extraction. On-column RNA clean-up, including a DNase digestion step, was performed using the RNeasy-Mini-Kit (Qiagen).
Label biotin
Label protocol Standard Affymetrix protocol.
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description X. laevis embryos, suv420 knockdown, injected side, replicate 2.
Data processing R/Bioconductor, gcrma normalization, default parameters.
Submission date Oct 01, 2012
Last update date Feb 06, 2013
Contact name Tobias Straub
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address GroƟhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL10756
Series (1)
GSE41256 Suv4-20h Histone Methyltransferases Promote Neuroectodermal Differentiation by Silencing the Pluripotency-Associated Oct-25 Gene

Data table header descriptions
VALUE GC-RMA expression value

Data table
AFFX-BioB-3_at 8.548134505
AFFX-BioB-5_at 8.153618103
AFFX-BioB-M_at 8.923842711
AFFX-BioC-3_at 10.27196448
AFFX-BioC-5_at 9.631948887
AFFX-BioDn-3_at 12.20122251
AFFX-BioDn-5_at 11.66305296
AFFX-CreX-3_at 14.18013052
AFFX-CreX-5_at 13.99949477
AFFX-DapX-3_at 11.97279422
AFFX-DapX-5_at 8.887060475
AFFX-DapX-M_at 10.70630438
AFFX-LysX-3_at 8.731185622
AFFX-LysX-5_at 6.654184878
AFFX-LysX-M_at 7.293500701
AFFX-PheX-3_at 8.602886957
AFFX-PheX-5_at 7.108007598
AFFX-PheX-M_at 8.013697824
AFFX-r2-Bs-dap-3_at 11.97882816
AFFX-r2-Bs-dap-5_at 9.270419984

Total number of rows: 32635

Table truncated, full table size 947 Kbytes.

Supplementary file Size Download File type/resource
GSM1012273_S_MO_80_INJ_Exp2.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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