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Sample GSM1016832 Query DataSets for GSM1016832
Status Public on Oct 10, 2012
Title Genistein diet, biological rep4
Sample type RNA
 
Source name Genistein diet
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
gender: male
tissue: liver
age: Experiment was started at 4 wks of age, rats were adapted to the condition for 5 days and fed the diet indicated for 14 days.
diet: containing 2 g/kg of Genistein
Treatment protocol Three groups of rats were fed an experimental diet containing 2 g/kg of either genistein or daidzein, or a control diet free of isoflavone for 14 days. The basal composition of the experimental diet was (in g/kg): casein, 200; palm oil, 100; corn starch, 150; cellulose, 20; mineral mixture (AIN-93G), 35; vitamin mixture (AIN-93), 10; L-cystine, 3.0; choline bitartrate, 2.0 and sucrose to 1 kg.
Growth protocol Male Sprague Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 weeks of age. The animals were housed individually in a room with controlled temperature and humidity and a 12 h light dark cycle.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen, Valencia, CA)
Label biotin
Label protocol One-Cycle Target Labeling and Control Reagents (Affymetrix)
 
Hybridization protocol GeneChip® Hybridization Oven 640 (Affymetrix)
Scan protocol GeneChip® Scanner 3000 (Affymetrix)
Description Genistein 6
Data processing The perfect match data were normalized by using the SuperNORM data service (Skylight Biotech Inc., Akita, Japan) according to a three-parameter lognormal distribution model [details on PMID 18764774, 18958174, 22784577]. The log values are log10 (rawdata - gamma): z-score = (log10 (rawdata- gamma) - mu)/sigma.
 
Submission date Oct 09, 2012
Last update date Oct 10, 2012
Contact name Tomokazu Konishi
E-mail(s) konishi@akita-pu.ac.jp
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
 
Platform ID GPL1355
Series (1)
GSE41420 A comparative analysis of genistein and daidzein in affecting lipid metabolism in rat liver

Data table header descriptions
ID_REF
VALUE parametric: z-score of logarithms.

Data table
ID_REF VALUE
1367452_at 1.537642178
1367453_at 1.455978059
1367454_at 1.684824058
1367455_at 1.901459186
1367456_at 2.322982829
1367457_at 1.035060766
1367458_at 1.055866578
1367459_at 2.417928739
1367460_at 2.111040639
1367461_at 1.465707401
1367462_at 1.563490359
1367463_at 2.004914818
1367464_at 1.182343384
1367465_at 1.600476958
1367466_at 1.426915291
1367467_at 1.874417696
1367468_at 1.463825501
1367469_at 2.031001391
1367470_at 1.476693471
1367471_at 1.22237135

Total number of rows: 31099

Table truncated, full table size 711 Kbytes.




Supplementary file Size Download File type/resource
GSM1016832_112006_Rat_Liver_Genistein_6.CEL.gz 2.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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