|
| Status |
Public on Mar 08, 2013 |
| Title |
KO-XY-1 |
| Sample type |
RNA |
| |
|
| Source name |
E18.5 Tex19.1-/- XY placenta
|
| Organism |
Mus musculus |
| Characteristics |
tissue: placenta
strain: C57BL/6
developmental stage: E18.5
gender: male
genotype/variation: Tex19.1-/-
|
| Treatment protocol |
None
|
| Growth protocol |
Freshly dissected material
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen), and then treated with DNase I. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
3.5
|
| Data processing |
Background subtracted (method=bgAdjust), log-transformed (method=”log2”), quantile-normalized (method=”quantile”) using lumi in R.
|
| |
|
| Submission date |
Oct 24, 2012 |
| Last update date |
Mar 08, 2013 |
| Contact name |
Ian Adams |
| Organization name |
MRC Human Genetics Unit
|
| Department |
Genome Regulation
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH42XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE41823 |
Gene expression in E18.5 Tex19.1-/- mouse placenta |
|
