|
Status |
Public on Mar 08, 2013 |
Title |
WT-XY-2 |
Sample type |
RNA |
|
|
Source name |
E18.5 Tex19.1+/+ XY placenta
|
Organism |
Mus musculus |
Characteristics |
tissue: placenta strain: C57BL/6 developmental stage: E18.5 gender: male genotype/variation: Tex19.1+/+
|
Treatment protocol |
None
|
Growth protocol |
Freshly dissected material
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen), and then treated with DNase I. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion).
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
2.5
|
Data processing |
Background subtracted (method=bgAdjust), log-transformed (method=”log2”), quantile-normalized (method=”quantile”) using lumi in R.
|
|
|
Submission date |
Oct 24, 2012 |
Last update date |
Mar 08, 2013 |
Contact name |
Ian Adams |
Organization name |
MRC Human Genetics Unit
|
Department |
Genome Regulation
|
Street address |
Crewe Road
|
City |
Edinburgh |
ZIP/Postal code |
EH42XU |
Country |
United Kingdom |
|
|
Platform ID |
GPL6887 |
Series (1) |
GSE41823 |
Gene expression in E18.5 Tex19.1-/- mouse placenta |
|