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Sample GSM1026857 Query DataSets for GSM1026857
Status Public on Mar 08, 2013
Title GBM7_GSC line_2
Sample type genomic
 
Channel 1
Source name Glioma Stem Cell line
Organism Homo sapiens
Characteristics cell line: GBM7
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega).
Label Cy3
Label protocol 1µg of genomic DNA was labeled according to the Agilent guidelines (www.agilent.com). Sample DNA was labeled with Cy3 and the Human Genomic DNA: Male (Promega), used as reference DNA, was labeled with Cy5.
 
Channel 2
Source name Human Genomic DNA: Male (Promega)
Organism Homo sapiens
Characteristics sample type: Genomic DNA comes from multiple anonymous donors.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega).
Label Cy5
Label protocol 1µg of genomic DNA was labeled according to the Agilent guidelines (www.agilent.com). Sample DNA was labeled with Cy3 and the Human Genomic DNA: Male (Promega), used as reference DNA, was labeled with Cy5.
 
 
Hybridization protocol Hybridization was performed on Agilent-014950 Human Genome CGH microarray 4x44K, following manufacturer's instructions (www.agilent.com for details).
Scan protocol Briefly, the arrays were scanned at 2μm resolution using Agilent microarray scanner (Agilent Technologies, Santa Clara, CA, USA).
Data processing Data were analyzed using Feature Extraction v10.7 and Agilent Genomic Workbench v5.0 softwares. The Aberration Detection Method 2 (ADM2) algorithm was used to compute and assist the identification of aberrations for each sample.
 
Submission date Oct 26, 2012
Last update date Mar 08, 2013
Contact name Simona Baronchelli
Organization name University of Milan-Bicocca
Street address Via Cadore 48
City Monza
ZIP/Postal code 20900
Country Italy
 
Platform ID GPL8841
Series (1)
GSE41875 Genome variation profiling of Glioma Stem Cell lines

Data table header descriptions
ID_REF
VALUE processed log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
A_14_P112718 -0.43843392
A_14_P201353 0.53941983
A_14_P108353 -1.1318167
A_14_P129881 -0.4887736
A_14_P114030 -0.4331566
A_14_P139527 0.36650747
A_14_P118340 -0.025628127
A_14_P106890 -0.30062988
A_14_P122641 0.71045923
A_14_P107170 -0.16167583
A_14_P100766 0.15950885
A_14_P118104 -0.27938828
A_14_P117253 -0.15526421
A_14_P129407 -0.6121813
A_14_P200001 -0.53858435
A_14_P103811 0.3778822
A_14_P119666 -0.42179707
A_14_P100799 0.14658459
A_14_P121179 0.031016042
A_14_P115318 -0.043219246

Total number of rows: 42416

Table truncated, full table size 1004 Kbytes.




Supplementary file Size Download File type/resource
GSM1026857_GBM7_cnv.txt.gz 4.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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