|
| Status |
Public on Jan 01, 2007 |
| Title |
13296232 |
| Sample type |
other |
| |
|
| Channel 1 |
| Source name |
I21 infected 21 h
|
| Organism |
Homo sapiens |
| Characteristics |
I21
|
| Extracted molecule |
other |
| Extraction protocol |
Small RNA purification using MirVana (Ambion) protocol from Total RNA.
|
| Label |
Cy3
|
| Label protocol |
1 μg of each small RNA fraction was chemically labelled with fluorescein and biotin using the MICROMAX kit (Perkin Elmer # MPS545).
|
| |
|
| Channel 2 |
| Source name |
N21 non-infected 21 h
|
| Organism |
Homo sapiens |
| Characteristics |
N21
|
| Extracted molecule |
other |
| Extraction protocol |
Small RNA purification using MirVana (Ambion) protocol from Total RNA.
|
| Label |
Cy5
|
| Label protocol |
1 μg of each small RNA fraction was chemically labelled with fluorescein and biotin using the MICROMAX kit (Perkin Elmer # MPS545).
|
| |
|
| |
| Hybridization protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 6.0 analysis software.
|
| Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 6.0 analysis software.
|
| Description |
2005/10/07 - 13296232
|
| Data processing |
GenePix Pro 6.0 analysis software
|
| |
|
| Submission date |
Apr 04, 2006 |
| Last update date |
Jan 11, 2007 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL3241 |
| Series (1) |
| GSE4761 |
Identification of host miRNA regulated by HIV-1 |
|
| Data table header descriptions |
| ID_REF |
|
| F635_MEDIAN |
CH1 (F635) median fluorescence intensity |
| B635_MEDIAN |
CH1 (F635) background median fluorescence intensity |
| F532_MEDIAN |
CH2 (F532) median fluorescence intensity |
| B532_MEDIAN |
CH2 (F532) background median fluorescence intensity |
| VALUE |
same as UNF_VALUE but with flagged values removed |
| FLAGS |
Denotes which features met our filtering criterion.(-50:autoflag, -100:manual flag) |
| UNF_VALUE |
Normalized log2 ratio of means defined by CH1/CH2 |
