|
Status |
Public on Sep 07, 2014 |
Title |
H2AX_iPSC_4N_negative_Bcell3 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
iPS cell (Bcell3) genomic DNA
|
Organism |
Mus musculus |
Characteristics |
strain: 129Sv/C57 mixed background
|
Treatment protocol |
No treatment
|
Growth protocol |
culture on feeder with standard media; just before get DNA, split cells on gelatin coated plates.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted with Qiagen Dneasy Blood ans Tissue Kit (Cat No. 69504)
|
Label |
Cy5
|
Label protocol |
DNA samples were labled with Nimblegen's standard protocol
|
|
|
Channel 2 |
Source name |
ESC (s1b6a) genomic DNA
|
Organism |
Mus musculus |
Characteristics |
strain: 129Sv/C57 mixed background
|
Treatment protocol |
No treatment
|
Growth protocol |
culture on feeder with standard media; just before get DNA, split cells on gelatin coated plates.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted with Qiagen Dneasy Blood ans Tissue Kit (Cat No. 69504)
|
Label |
Cy3
|
Label protocol |
DNA samples were labled with Nimblegen's standard protocol
|
|
|
|
Hybridization protocol |
Labeled DNA hybridized to CGH array with Nimblegen's standard protocol
|
Scan protocol |
Arrays were scaned with Nimblegen's standard protocol
|
Description |
iPSC_Bcell3_vs_ESC CGH
|
Data processing |
Arrays were processed with Nimblegen's standard protocol
|
|
|
Submission date |
Nov 13, 2012 |
Last update date |
Sep 08, 2014 |
Contact name |
Andrew Zhuo Xiao |
E-mail(s) |
andrew.xiao@yale.edu
|
Phone |
203-785-5111
|
Organization name |
Yale Stem Cell Center
|
Department |
Genetics
|
Lab |
Andrew Xiao
|
Street address |
10 Amistad St
|
City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06519 |
Country |
USA |
|
|
Platform ID |
GPL15225 |
Series (2) |
GSE42266 |
CNV analysis of iPS cell lines and H2A.X knockout ES cell |
GSE42309 |
H2A.X function in mouse ESC and iPSC (ChIPSeq and CNVs) |
|