|
Status |
Public on Apr 21, 2015 |
Title |
NFH-46, replicate 1 |
Sample type |
RNA |
|
|
Source name |
derma
|
Organism |
Homo sapiens |
Characteristics |
cell line: NFH46 age: 81 year old woman, passage 4 tissue: dermal fibroblasts
|
Extracted molecule |
total RNA |
Extraction protocol |
The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
Label |
biotin
|
Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 1
|
Data processing |
Illumina BeadStudio software version 3
|
|
|
Submission date |
Nov 26, 2012 |
Last update date |
Apr 21, 2015 |
Contact name |
Alessandro Prigione |
E-mail(s) |
alessandro.prigione@mdc-berlin.de
|
Organization name |
Max Delbrueck Center for Molecular Medicine
|
Lab |
Department of Neuroproteomics
|
Street address |
Robert-Roessle-Str. 10
|
City |
Berlin |
State/province |
Berlin |
ZIP/Postal code |
D-13125 |
Country |
Germany |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE42492 |
Induced pluripotent stem cell-derived neurons from a sporadic Alzheimer disease donor as a model for investigating disease mechanisms |
|