|
| Status |
Public on Oct 01, 2015 |
| Title |
35S_GLK1-SRDX_Arabidopsis_2wks_seedling-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
35S:GLK1-SRDX Arabidopsis thaliana 2wks seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype: 35S:GLK1-SRDX
age: 2wks
tissue: whole seedlings
|
| Treatment protocol |
None
|
| Growth protocol |
All plants were grown in MS (1% sucrose) agar medium in a 16hr light / 8hr dark cycle in 23 Celsius degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.
|
| Label |
Cy3
|
| Label protocol |
1 ug of total RNA was used for the labeling procedure using the Quick Amp Labeling kit (One-color) (Agilent).
|
| |
|
| Hybridization protocol |
Hybridized using the Gene Expression Hybridization kit (Agilent) and washed by the Gene Expression wash pack (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
|
| Description |
GLK1SRDX_rep3
Many seedlings were grown in MS agar medium and were harvested for RNA extraction.
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction. After that, the signal of each probe was divided by the median value of filter-passed probes.
|
| |
|
| Submission date |
Nov 27, 2012 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
nobutaka.mitsuda@aist.go.jp
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Bioproduction Research Institute
|
| Lab |
Gene Regulation Research Group
|
| Street address |
Central 6 Higashi 1-1-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7299 |
| Series (1) |
| GSE42545 |
Chimeric repressors of GLKs confer tolerance to air pollutant by closing stomata in Arabidopsis |
|
