|
| Status |
Public on Dec 05, 2012 |
| Title |
BMDC_ID8 |
| Sample type |
RNA |
| |
|
| Source name |
BMDC_tumor cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Bone Marrow-derived Dendritic Cells (BMDCs)
co-cultured with: ID8 tumor cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using the TRIZOL® Reagent(Invitrogen) according to manufacturer’s instructions.
|
| Label |
Hy3
|
| Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Cat #208032-A, Exiqon) according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The labeled samples were hybridized on the miRCURY LNA™ microRNA Array V11.0.
|
| Scan protocol |
Hybridization signals were detected by fluorescence using an GenePix 4000B scanner (Molecular Devices). Scanner images were quantified by GenePix Pro 6 (Molecular Devices).
|
| Description |
RY2009_02
|
| Data processing |
For each spot, verify the signal by subtracting background intensity (B) from foreground intensity (F). Verified signal value= F - B. The spots, of which verified signal values are less than 50, and the control spots are filtered. For verified signal values of the left spots, perform median normalization.
|
| |
|
| Submission date |
Dec 04, 2012 |
| Last update date |
Dec 05, 2012 |
| Contact name |
Yang Rongcun |
| E-mail(s) |
ryang@nankai.edu.cn
|
| Phone |
+86-(22)-23509007
|
| Fax |
+86-(22)-23502554
|
| Organization name |
Nankai University
|
| Department |
School of medicine
|
| Street address |
94 Weijin Road, Nankai District
|
| City |
Tianjin, P.R.China |
| ZIP/Postal code |
300071 |
| Country |
China |
| |
|
| Platform ID |
GPL16352 |
| Series (1) |
| GSE42722 |
miRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells |
|
