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Sample GSM1048763 Query DataSets for GSM1048763
Status Public on Dec 05, 2012
Title BMDC_ID8
Sample type RNA
Source name BMDC_tumor cells
Organism Mus musculus
Characteristics cell type: Bone Marrow-derived Dendritic Cells (BMDCs)
co-cultured with: ID8 tumor cells
Extracted molecule total RNA
Extraction protocol Total RNAs were isolated using the TRIZOL® Reagent(Invitrogen) according to manufacturer’s instructions.
Label Hy3
Label protocol The samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Cat #208032-A, Exiqon) according to manufacturer's instructions.
Hybridization protocol The labeled samples were hybridized on the miRCURY LNA™ microRNA Array V11.0.
Scan protocol Hybridization signals were detected by fluorescence using an GenePix 4000B scanner (Molecular Devices). Scanner images were quantified by GenePix Pro 6 (Molecular Devices).
Description RY2009_02
Data processing For each spot, verify the signal by subtracting background intensity (B) from foreground intensity (F). Verified signal value= F - B. The spots, of which verified signal values are less than 50, and the control spots are filtered. For verified signal values of the left spots, perform median normalization.
Submission date Dec 04, 2012
Last update date Dec 05, 2012
Contact name Yang Rongcun
Phone +86-(22)-23509007
Fax +86-(22)-23502554
Organization name Nankai University
Department School of medicine
Street address 94 Weijin Road, Nankai District
City Tianjin, P.R.China
ZIP/Postal code 300071
Country China
Platform ID GPL16352
Series (1)
GSE42722 miRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
1001 11.86001255
1002 0.005021971
1003 0.12868801
1004 -0.005649718
1005 -0.001883239
1006 0.13747646
1007 -0.005021971
1008 0.355304457
1009 0.018832392
1010 -0.001255493
1011 0.046453233
1012 0.001255493
1013 0
1014 0.860012555
1015 0.010671689
1016 12.09730069
1017 0.008788449
1018 0.02259887
1019 0.020087884
1020 0.011927181

Total number of rows: 1408

Table truncated, full table size 23 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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