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Sample GSM1053605 Query DataSets for GSM1053605
Status Public on Aug 15, 2013
Title mosaic larval eye-antennal discs RasACT overexpressing scrib mutant 1
Sample type RNA
 
Source name RasV12 expressing scrib mutant Drosophila larval mosaic eye-antennal discs
Organism Drosophila melanogaster
Characteristics tissue: larval mosaic eye-antennal discs
genotype: RasV12 expressing scrib mutant
developmental stage: 3rd instar larvae
genetic background: w1118
Treatment protocol eye-annennal disc were dissected from 3rd instar larvae at day 5 after egg deposition.
Growth protocol Larvae were incubated at 25oC and grown on standard fly media
Extracted molecule total RNA
Extraction protocol Samples were prepared in triplicate, and the RNA isolated using TRIZOL, before being column purified (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
 
Hybridization protocol Hybridization was carried out following standard Affymetrix protocols
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 following Affymetrix protocols.
Description tumours
Data processing The data was gcrma normalised using the R Bioconductor package gcrma.
 
Submission date Dec 14, 2012
Last update date Aug 15, 2013
Contact name Helena Elizabeth Richardson
E-mail(s) helena.richardson@petermac.org
Phone 61396561466
Organization name Peter MacCallum Cancer Center
Department Research Division
Lab Cell Cycle and Development lab
Street address 7 St Andrew's place
City East Melbourne
State/province Victoria
ZIP/Postal code 3002
Country Australia
 
Platform ID GPL1322
Series (1)
GSE42938 Abrupt scrib- vs. Abrupt and RasV12 (RasACT) scrib-, NotchICD (NACT) scrib- +/- JNK (Bsk) expression profiles

Data table header descriptions
ID_REF
VALUE Log base 2 gcrma normalised expression value

Data table
ID_REF VALUE
1616608_a_at 9.5442852
1622892_s_at 8.29252548
1622893_at 2.174348133
1622894_at 2.174348133
1622895_at 10.11583978
1622896_at 8.027300068
1622897_at 2.174348133
1622898_a_at 10.9581376
1622899_at 4.220337264
1622900_at 2.174348133
1622901_at 2.18999655
1622902_at 2.174348133
1622903_s_at 7.856334321
1622904_at 2.174348133
1622905_at 2.176661943
1622906_at 9.044434565
1622907_at 8.818730988
1622908_a_at 2.388975477
1622909_at 9.46293765
1622910_at 2.174348133

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM1053605_RasACTscrib1.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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