RNA was extracted using Triazol reagent (Invitrogen Corporation, Carlsbad, CA, USA) according to the manufacturer’s instructions. DNase I (Invitrogen Corporation, Carlsbad, CA, USA) treatment was performed according to the manufacturer’s instructions
Label
Cy3
Label protocol
Cy3-streptavidin (Amersham Biosciences, Little Chalfont, United Kingdom) as recommended by the manufacturer
Hybridization protocol
0.75 ug of total RNA was amplified using Illumina TotalPrep RNA amplification kit (Applied Biosystems, Foster City, CA, USA). cRNA was hybridized to Illumina Human-6 Expression BeadChip (Illumina, San Diego, CA, USA) as recommended by the manufacturer
Scan protocol
The arrys were scanned by the Bead Station (Illumina, San Diego, CA, USA) as recommended by the manufacturer
Description
SAMPLE 2
Data processing
Array data processing was performed with Illumina BeadStudio software.
