NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1055410 Query DataSets for GSM1055410
Status Public on Jun 30, 2015
Title Chronic Nasopharyngitis tissue 13
Sample type RNA
 
Channel 1
Source name human biopsy of chronic nasopharyngitis
Organism Homo sapiens
Characteristics biopsy: human biopsy of chronic nasopharyngitis
diagnosis: chronic nasopharyngitis
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Hy5
Label protocol 6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
 
Channel 2
Source name Total RNA from Human lung fibroblasts cells
Organism Homo sapiens
Characteristics cell line: HLF
passage: 6
cell type: HLF drived from Human lung fibroblasts
Growth protocol HLF cells were cultured in RPMI-1640 with 10% Fetal Cow Serum
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Hy3
Label protocol 6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
 
 
Hybridization protocol Oligoarray control targets and hybridization buffer were added, and samples were applied to microarrays enclosed in LF-I molecular hybridization chambers(Scientz, China). After hybridization, slides were washed sequential
Scan protocol Scanned on an CapitalBio LuxScan 10K scanner.
Images were quantified using Genepix pro Software (version 6.0).
Description Biological replicate 13 of 20. Chronic Nasopharyngitis tissue 13
CNP13
Data processing LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Madb and BRB ArrayTools were used.
 
Submission date Dec 19, 2012
Last update date Jun 30, 2015
Contact name Xin Li
E-mail(s) xinli268@gmail.com
Phone 86 20 62789438
Organization name Southern medical university
Department Cancer research institute
Street address Southern medical university
City Guangzhou
State/province Province
ZIP/Postal code 510515
Country China
 
Platform ID GPL16414
Series (2)
GSE42945 The novel functional effects of exogenous EBV-miR-BARTs on NPC progression
GSE43039 microRNA expression profiling of human nasopharyngeal Carcinoma tissues and Chronic Nasopharyngitis specimens

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Hy5/Hy3) representing test/reference

Data table
ID_REF VALUE
1_6519882 0.4
2_6519882 9.296
1_6519883 -1.004
2_6519883 0.456
1_6519884 -3.198
2_6519884 9.296
1_6519885 -1.257
2_6519885 -1.172
1_6519886 1.868
2_6519886 -0.16
1_6519887 1.246
2_6519887 1.195
1_6519888 -0.406
2_6519888 -0.473
1_6519889 -1.256
2_6519889 -1.002
1_6519890 0.104
2_6519890 0.033
1_6519891 1.211
2_6519891 1.213

Total number of rows: 1712

Table truncated, full table size 27 Kbytes.




Supplementary file Size Download File type/resource
GSM1055410_CNP13.gpr.gz 213.5 Kb (ftp)(http) GPR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap