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Sample GSM1055414 Query DataSets for GSM1055414
Status Public on Jun 30, 2015
Title Chronic Nasopharyngitis tissue 17
Sample type RNA
 
Channel 1
Source name human biopsy of chronic nasopharyngitis
Organism Homo sapiens
Characteristics biopsy: human biopsy of chronic nasopharyngitis
diagnosis: chronic nasopharyngitis
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Hy5
Label protocol 6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
 
Channel 2
Source name Total RNA from Human lung fibroblasts cells
Organism Homo sapiens
Characteristics cell line: HLF
passage: 6
cell type: HLF drived from Human lung fibroblasts
Growth protocol HLF cells were cultured in RPMI-1640 with 10% Fetal Cow Serum
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Hy3
Label protocol 6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
 
 
Hybridization protocol Oligoarray control targets and hybridization buffer were added, and samples were applied to microarrays enclosed in LF-I molecular hybridization chambers(Scientz, China). After hybridization, slides were washed sequential
Scan protocol Scanned on an CapitalBio LuxScan 10K scanner.
Images were quantified using Genepix pro Software (version 6.0).
Description Biological replicate 17 of 20. Chronic Nasopharyngitis tissue 17
CNP17
Data processing LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Madb and BRB ArrayTools were used.
 
Submission date Dec 19, 2012
Last update date Jun 30, 2015
Contact name Xin Li
E-mail(s) xinli268@gmail.com
Phone 86 20 62789438
Organization name Southern medical university
Department Cancer research institute
Street address Southern medical university
City Guangzhou
State/province Province
ZIP/Postal code 510515
Country China
 
Platform ID GPL16414
Series (2)
GSE42945 The novel functional effects of exogenous EBV-miR-BARTs on NPC progression
GSE43039 microRNA expression profiling of human nasopharyngeal Carcinoma tissues and Chronic Nasopharyngitis specimens

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Hy5/Hy3) representing test/reference

Data table
ID_REF VALUE
1_6519882 -1.885
2_6519882 0.386
1_6519883 11.72
2_6519883 0.132
1_6519884 -1.383
2_6519884 -0.979
1_6519885 -1.482
2_6519885 -1.448
1_6519886 0.46
2_6519886 0.584
1_6519887 1.137
2_6519887 1.257
1_6519888 -0.713
2_6519888 -0.827
1_6519889 -1.11
2_6519889 -1.114
1_6519890 -0.261
2_6519890 -0.477
1_6519891 2.022
2_6519891 3.676

Total number of rows: 1712

Table truncated, full table size 27 Kbytes.




Supplementary file Size Download File type/resource
GSM1055414_CNP17.gpr.gz 214.5 Kb (ftp)(http) GPR
Processed data included within Sample table

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