|
Status |
Public on Jun 30, 2015 |
Title |
Chronic Nasopharyngitis tissue 17 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
human biopsy of chronic nasopharyngitis
|
Organism |
Homo sapiens |
Characteristics |
biopsy: human biopsy of chronic nasopharyngitis diagnosis: chronic nasopharyngitis
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Hy5
|
Label protocol |
6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
|
|
|
Channel 2 |
Source name |
Total RNA from Human lung fibroblasts cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: HLF passage: 6 cell type: HLF drived from Human lung fibroblasts
|
Growth protocol |
HLF cells were cultured in RPMI-1640 with 10% Fetal Cow Serum
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Hy3
|
Label protocol |
6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
|
|
|
|
Hybridization protocol |
Oligoarray control targets and hybridization buffer were added, and samples were applied to microarrays enclosed in LF-I molecular hybridization chambers(Scientz, China). After hybridization, slides were washed sequential
|
Scan protocol |
Scanned on an CapitalBio LuxScan 10K scanner. Images were quantified using Genepix pro Software (version 6.0).
|
Description |
Biological replicate 17 of 20. Chronic Nasopharyngitis tissue 17 CNP17
|
Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Madb and BRB ArrayTools were used.
|
|
|
Submission date |
Dec 19, 2012 |
Last update date |
Jun 30, 2015 |
Contact name |
Xin Li |
E-mail(s) |
xinli268@gmail.com
|
Phone |
86 20 62789438
|
Organization name |
Southern medical university
|
Department |
Cancer research institute
|
Street address |
Southern medical university
|
City |
Guangzhou |
State/province |
Province |
ZIP/Postal code |
510515 |
Country |
China |
|
|
Platform ID |
GPL16414 |
Series (2) |
GSE42945 |
The novel functional effects of exogenous EBV-miR-BARTs on NPC progression |
GSE43039 |
microRNA expression profiling of human nasopharyngeal Carcinoma tissues and Chronic Nasopharyngitis specimens |
|