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Sample GSM106830 Query DataSets for GSM106830
Status Public on Mar 01, 2010
Title Saccharomyces cerevisiae gcn5 mutant KCl treatment replicate 2
Sample type RNA
 
Channel 1
Source name Saccharomyces cerevisiae gcn5 mutant strain 17285 treated with 1M KCl for 60min.
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae gcn5 mutant strain 17285 treated with 1M KCl for 60min at 25C.
Treatment protocol Saccharomyces cerevisiae gcn5 mutant strain 17285 treated with 1M KCl for 60min at 25C.
Growth protocol Strain was grown on YPD medium at 25C to log phase then treated with 1M KCl for 60min.
Extracted molecule total RNA
Extraction protocol See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004)
Label cy3
Label protocol See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004)
 
Channel 2
Source name Saccharomyces cerevisiae wild type strain By4742 treated with 1M KCl for 60min.
Organism Saccharomyces cerevisiae
Characteristics Saccharomyces cerevisiae wild type strain By4742 treated with 1M KCl for 60min at 25C as control.
Treatment protocol Saccharomyces cerevisiae wild type strain By4742 treated with 1M KCl for 60min at 25C as control.
Growth protocol Strain was grown on YPD medium at 25C to log phase then treated with 1M KCl for 60min.
Extracted molecule total RNA
Extraction protocol See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
Label cy5
Label protocol See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
 
 
Hybridization protocol See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
Scan protocol Slides were scanned using Bio-rad Vers-Array ChipReader.
Description Saccharomyces cerevisiae gcn5 mutant strain 17285 treated with 1M KCl for 60min compared to wild type parental strain By4742 treated with similar condition.
Data processing Scanned slides were quantified with Imagene 4.2 software. Quantified data were loaded to GeneSpring software and normalized by lowess normalization.
 
Submission date Apr 27, 2006
Last update date Jan 08, 2010
Contact name Anthony Wright
E-mail(s) yongtao.xue.franzen@ki.se
Organization name Karolinska Institute
Lab Anthony Wright
Street address Blickagången 6
City Huddinge
ZIP/Postal code 141 57
Country Sweden
 
Platform ID GPL3690
Series (2)
GSE5218 Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe
GSE16556 Role of Gcn5 in KCl stress adaptation in S. cerevisiae and S. pombe: transcriptional and genomic analyses

Data table header descriptions
ID_REF
VALUE-non log Ratio of the test group compared to control group
VALUE log2 ratio of the test group compared to control grou
NormCH1 Normalized signal of test group
NormCH2 Normalized singal of control group
Flag Indicating if spot quality, P: good spot, A: spot with absent or poor signal
Raw test signal Raw signal of test group
Raw test background Raw signal backgroud of test group
Raw control signal Raw signal of control group
Raw control background Raw signal backgroud of control group

Data table
ID_REF VALUE-non log VALUE NormCH1 NormCH2 Flag Raw test signal Raw test background Raw control signal Raw control background
1 A 70.19 75.87368421 244.6 233.5157895
2 A 82.09375 85.45801527 295.796875 259.8778626
3 A 100.1090909 85.14529915 314.8181818 297.8803419
4 A 79.61 90.56097561 276.17 238.4146341
5 A 73.29090909 76.48201439 174.6545455 244.9856115
6 A 111.4567901 80.68148148 231.8271605 237.437037
7 A 80.2 52.98591549 256.27 231.8169014
8 A 62.16363636 77.62237762 285.1636364 228.1398601
9 A 116.9753086 73.87735849 302.9012346 192.3962264
10 A 61.6 73.73426573 207.3272727 264.4685315
11 P 312.87 46.37142857 696.74 216.7619048
12 A 243.44 77.75675676 714.03 277.5945946
13 P 417.46 84.12121212 752.29 242.5757576
14 P 264.47 97.88043478 776.35 251.4021739
15 P 886.54 68.52173913 1853.42 214.7391304
16 P 590.5 79.87878788 1174.95 300.6666667
17 P 910.96 85.74390244 1815.67 217.4390244
18 P 940.55 82.28 1920.06 230.7733333
19 P 1585.97 62.57024793 2759.62 272.7107438
20 P 1072.41 74.2826087 2072.58 288.9130435

Total number of rows: 12800

Table truncated, full table size 865 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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