|
| Status |
Public on Jun 16, 2014 |
| Title |
µ0.1 rep1 8 min |
| Sample type |
RNA |
| |
|
| Source name |
chemostat 0.1 h-1 repet1 4min
|
| Organism |
Lactococcus lactis |
| Characteristics |
strain: IL1402
treatment: µ0.1 rifampicin
time: 8 min
|
| Treatment protocol |
At T=0 rifampicin was added into the culture at a final concentration of 500 µg.mL-1 so as to block transcription, mRNA levels were followed during the next 20 minutes
|
| Growth protocol |
cultures were performed in chemically defined medium with low Branched-Chain Amino Acid concentrations. Chemostat cultures (growth rates of 0.51 and 0.11h-1) were isoleucine limited whereas isoleucine starvation occured progressively in batch cultures (grawth rates of 0.8, 0.38 and 0.04 h-1).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385
|
| Label |
32P
|
| Label protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385
|
| |
|
| Hybridization protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385
|
| Scan protocol |
see Redon et al. 2005. Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation. J Biol Chem 280(43):36380-385
|
| Data processing |
Raw data were processed to access mRNA concentrations and were normalized to the mean intensity of the reference membrane (before rifampicin addition) for each batch. mRNA half-lives (t1/2) were calculated from the degradation rate constant (k), which corresponds to the slope of mRNA concentration logarithm as a function of time with the relation t1/2=ln(2)/k
|
| |
|
| Submission date |
Jan 29, 2013 |
| Last update date |
Jun 16, 2014 |
| Contact name |
Muriel Cocaign-Bousquet |
| E-mail(s) |
cocaign@insa-toulouse.fr
|
| URL |
http://www.insa-toulouse.fr/fr/recherche/labo/lisbp.html
|
| Organization name |
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
|
| Department |
UMR5504 INSA/CNRS - UMR792 INSA/INRA
|
| Street address |
135 avenue de Rangueil
|
| City |
Toulouse |
| ZIP/Postal code |
31077 |
| Country |
France |
| |
|
| Platform ID |
GPL16567 |
| Series (1) |
| GSE43875 |
Role of mRNA stability during bacterial adaptation |
|
