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Sample GSM1074275 Query DataSets for GSM1074275
Status Public on Feb 04, 2014
Title P53_nor_chipN_TileShuffle
Sample type RNA
 
Source name Colorectal carcinoma cells with defunct P53 (D53wt cells)
Organism Homo sapiens
Characteristics cell type: colorectal carcinoma cells with defunct P53 (D53wt cells)
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol 10µg RNA were analyzed on Affymetrix Human Tiling 1.0 array set according to the manufacturer's protocol.
 
Hybridization protocol Affymetrix Human Tiling 1.0 arrays were proceed using the GCS3000 7G system according to manufacturer's protocol.
Scan protocol Affymetrix Human Tiling 1.0 array were proceed using the GCS3000 7G system according to manufacturer's protocol.
Description Whole transcriptome expression analysis of colorectal carcinoma cells with defunct P53 on Affymetrix Human Tiling 1.0 array set. Intensities were processed with TileShuffle.
Data processing Affymetrix Human Tiling 1.0 probes were mapped to human genome assembly hg18 using the corresponding BPMAP files. Intensities of perfect match (PM) probes were processed with TileShuffle (Otto C, Reiche K, Hackermüller J. "Detection of differentially expressed segments in tiling array data". Bioinformatics. 2012 Jun 1;28(11):1471-9. Epub 2012 Apr 6.) using a window size of 200, the arithmetic mean trimmed by maximal and minimal value as scoring function, four GC content bins, 10000 permutations, and a q-value threshold of 0.05. In order to estimate the significance of a window score we repeatedly permute all probe intensities across the array while interchanging only those that belong to the same sequence-specific affinity bin, recompute the window scores, and compare them with the original ones. By counting the number of permuted windows with higher score, we estimate empirical p-values of windows, and report q-values adjusted according to Benjamini and Hochberg ("Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing". Journal of the Royal Statistical Society. Series B (Methodological), 57:289-300, 1995.).
 
Submission date Jan 30, 2013
Last update date Feb 05, 2014
Contact name Kristin Reiche
E-mail(s) kristin.reiche@izi.fraunhofer.de
Organization name Fraunhofer Institute for Cell Therapy and Immunology
Department Diagnostics
Street address Perlickstr. 1
City Leipzig
ZIP/Postal code 04103
Country Germany
 
Platform ID GPL6169
Series (2)
GSE43912 Expression analysis of colorectal carcinoma cells with defunct P53 (D53wt cells)
GSE43914 Differential expression analysis of colorectal carcinoma cells (D53wt)

Supplementary file Size Download File type/resource
GSM1074275_P53_nor_chipN.CEL.gz 20.2 Mb (ftp)(http) CEL
GSM1074275_P53_nor_chipN_high_.bed.gz 30.9 Kb (ftp)(http) BED
Processed data provided as supplementary file

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