|
| Status |
Public on Feb 07, 2013 |
| Title |
HIV-1 Vpr (V2)_SH-SY5Y [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
Human Neuronal Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SH-SY5Y neuronal cell line
treatment: HIV-1 Vpr
|
| Treatment protocol |
Cells were treated with 10 pg/ml of Vpr protein kindly received from Dr Eric Cohen, University of Montreal-Canada or with 10 ng/ml of Tat protein kindly received from NIH (AIDS reagnets Bank) for 24 hours.
|
| Growth protocol |
Neuronal cell line, SH-SY5Y (5 x 105) were grown in DMEM + 10% FBS on MatTek glass bottom plates treated with collagen (MatTek, Ashland, MA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol (Invitrogen, Carlsbad, CA). 750 ng of total RNA was used for miRNA array analysis using miRCURY LNATM microRNA Array, v. 11.0 (Exiqon, Woburn, MA).
|
| Label |
Hy5
|
| Label protocol |
Standard Agilent protocol.
|
| |
|
| Hybridization protocol |
Standard Agilent protocol.
|
| Scan protocol |
Array chips were scanned using Axon GenePix Scanner (Molecular Devices, Downingtown, PA) and Genepix 4000 image capture software (Molecular Devices, Downingtown, PA).
|
| Data processing |
Quantile normalization was used using JMP Genomics (JMP, Cary, NC) at Harvard Catalyst - Laboratory for Innovative Translational Technology (HC-LITT) and differentially regulated miRNAs were determined. Untreated cells were used as control.
|
| |
|
| Submission date |
Feb 07, 2013 |
| Last update date |
Feb 12, 2013 |
| Contact name |
Bassel E Sawaya |
| E-mail(s) |
sawaya@temple.edu
|
| Phone |
2157075446
|
| Organization name |
Temple University
|
| Department |
Neurology
|
| Street address |
3307 N. Broad Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
| |
|
| Platform ID |
GPL8227 |
| Series (1) |
| GSE44266 |
Deregulation of microRNAs by HIV-1 Vpr protein leads to the development of neurocognitive disorders. |
|
