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Sample GSM1081537 Query DataSets for GSM1081537
Status Public on Dec 16, 2013
Title mRNA-seq, RAD21cv HEK293, HRV treated, replicate two
Sample type SRA
Source name mRNA-seq, RAD21cv HEK293, HRV treated
Organism Homo sapiens
Characteristics cell line: HEK293T RAD21cv
Treatment protocol A stably integrated transgene in the RAD21cv HEK293T cells was activated for 3 days with 2µg/mL of doxycycline. Cells were then transfected with a plasmid expressing HRV protease using Lipofectamine 2000 (Invitrogen) for 24 hours before harvesting
Growth protocol Cells were grown in DMEM supplemented with 0.2mM L-glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin, and 10% fetal calf serum at 37°C and 5% CO2
Extracted molecule polyA RNA
Extraction protocol Total RNA was extract using Trizol (Invitrogen) according to manufacturer's instructions. polyA RNA was isolated using Dynalbeads mRNA purification kit (Invitrogen) according to the manufacturer's instructions.
Strand Specific libraries were constructed according to Parkhomchuk et al. 2009, Nucleic Acids Res
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing fastq: Illumina's HiSeq Control Software
Hi-C reads were aligned as single end reads using BWA with default parameters. Uniquely mapping reads were manually paired using in an in house pipeline. PCR duplicate reads were removed using Picard MarkDuplicates.
RNA-seq data were mapped as paired-end reads using tophat with the following parameters: -g 1 -p 12 --solexa1.3-quals --library-type fr-firststrand --segment-length 25 --bowtie1
RNA-seq bigWig files were generated through an in house pipeline that includes performing trimmed mean of M (TMM) normalization (Robinson et al. Genome Biology 2010)
ChIP-seq data were mapping with bowtie using the following parameters: -v 3 -m 1 --best --strata -S --time -p 8. Data was filtered for uniquely mapping reads. PCR duplicates were removed using Picard MarkDuplicates
ChIP-seq bigWig files were generated through an in house pipeline
Genome_build: hg18
Supplementary_files_format_and_content: ChIP-seq and RNA-seq processed files are in bigWig format. As the RNA-seq experiment was strand specific, bigWig files for both the positive and negative strands are included
Supplementary_files_format_and_content: Hi-C processed files are in a modified bed format. Each row lists the chromosome and the start and end coordiates of two interacting bins as well as the normalized interaction frequency between these two bins
Supplementary_files_format_and_content: Processed mRNA-seq data include information for each gene and its count and rpkm values. Each line includes chromosome, start position, end position, strand, gene name, read counts, and rpkm values
Genome Build: hg18 hg18
HRV_r2.rpkm: hg18
Submission date Feb 12, 2013
Last update date May 15, 2019
Contact name Jesse R Dixon
Organization name Salk Institute for Biological Studies
Street address 10010 N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platform ID GPL11154
Series (1)
GSE44267 Cohesin and CTCF Differentially Affect the Chromatin Architecture and Gene Expression in Human Cells
SRA SRX235603
BioSample SAMN01919648

Supplementary file Size Download File type/resource 173.2 Mb (ftp)(http) BW 180.0 Mb (ftp)(http) BW
GSM1081537_HRV_r2.rpkm.gz 454.0 Kb (ftp)(http) RPKM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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