|
| Status |
Public on May 27, 2013 |
| Title |
BEAS2B-PDBu4h-2 |
| Sample type |
RNA |
| |
|
| Source name |
BEAS-2B cells after 4 hours of PDBu treatment
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Phorbol 12, 13-dibutyrate
time: 4 h
cell line: BEAS-2B
cell type: Immortalized Bronchial Epithelial Cell Culture
|
| Treatment protocol |
We stimulated BEAS-2B cells with 500 nM of PDBu for 30 minutes or 4 hours.
|
| Growth protocol |
Cells are cultured in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum. Cells were grown at 37°C with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit (Qiagen, Valencia, CA) was used to extract total RNA.
|
| Label |
biotin
|
| Label protocol |
RNA was prepared and labelled using the Ambion WT Expression Kit.
|
| |
|
| Hybridization protocol |
Human Gene 1.0 ST Arrays were prepared according to the FS450_0007 Fluidics Protocol (GeneChip® Whole Transcript Sense Target Labeling Assay Manual, Chapter 5)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
Affymetrix CEL files were imported into Partek Genomics Suite (Partek Inc., St. Louis, MO) and pre-processed using RMA with Partek's default RMA settings. PCA, Heirarchical Clustering and ANOVA were also performed in Partek Genomics Suite. Pathway and function enrichment analysis were performed with Igenuity Pathway Analysis (IPA) (Ingenuity Systems, Inc., Redwood City, CA) and DAVID (National Institute of Allergy and Infectious Diseases, NIH).
|
| |
|
| Submission date |
Feb 28, 2013 |
| Last update date |
May 28, 2013 |
| Contact name |
Ricardo Zamel |
| Organization name |
University Health Network
|
| Street address |
101 College St.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE44747 |
PKC Activation Induces Inflammatory Response and Cell Death in Human Bronchial Epithelial Cells |
|
