|
| Status |
Public on Mar 18, 2013 |
| Title |
HIF2α #17-56 kidney 2 |
| Sample type |
SRA |
| |
|
| Source name |
kidney cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
genotype: gamma-HIF2alphaM3 TG+
tissue: kidney cortex
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Animals were kept at standard conditions in Research Animal Resource Center of WCMC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Thin outer slice of kidney cortex was immediately removed from the animals. mRNA was extracted from the kidney cortex slice using mini-RNAeasy columns (Qiagen, Valencia, CA).
illumina TruSeq RNA Sample Preparation kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Reads were aligned to the genome using the Burrows-Wheeler Aligner in Goby web software (goby_1.9.8.3 (20120216154520)), using default parameters.
Differential Expression Analysis were done with Differential Expression Analysis with Goby in Goby web software (goby_1.9.8.3 (20120216154520)), using default parameters.
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using Goby Web software.
Genome_build: NCBI37.55/MM9
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Mar 18, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Leiping Fu |
| E-mail(s) |
lef2010@med.cornell.edu
|
| Phone |
212-746-6262
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Pharmacology
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE45254 |
mRNA profiling of GGT-HIF2aM3 (gamma-HIF2alphaM3) kidney cortex |
|
| Relations |
| SRA |
SRX251967 |
| BioSample |
SAMN01983937 |
