|
| Status |
Public on Mar 30, 2014 |
| Title |
Mature Root of R-Line, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Roots of 40-60-days-old plants
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
tissue: Root
genotype: R-line_PR319
cultivar: Indica
developmental stage: 40-60-days-old plants
|
| Growth protocol |
Rice seeds of B, H and R line were surface sterilized with 0.1% HgCl2 and were soaked in RO (reverse osmosis purified) water overnight in dark. Next day, the seeds were spread on a gauze float and grown hydroponically in RGM medium at 28±2 oC in culture room conditions. Shoots and roots were separated and frozen in liquid nitrogen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix fluidic station model 450 using protocol FS_450_0001
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 7000 (Affymetrix)
|
| Description |
Gene expression data of root tissue of 40-60-days-old palnt of R-line
|
| Data processing |
The data were analyzed with ArrayAssist Version 5.5 using default analysis settings and global scaling GCRMA as normalization method.
|
| |
|
| Submission date |
Apr 02, 2013 |
| Last update date |
Mar 30, 2014 |
| Contact name |
Sanjay Kapoor |
| E-mail(s) |
kapoors@genomeindia.org
|
| Phone |
91 11 65171135
|
| URL |
http://www.dpmb.ac.in/
|
| Organization name |
University of Delhi South Campus
|
| Department |
Department of Plant Molecular Biology
|
| Street address |
Benito Juarez Road
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE45718 |
Expression data from early and late vegetative stages from rice hybrid and its parents |
|
