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Sample GSM112132 Query DataSets for GSM112132
Status Public on Oct 01, 2006
Title MoSV stria vascularis MPSS library
Sample type MPSS
 
Source name mouse stria vascularis
Organism Mus musculus
Characteristics Strain: C57BL/6
Age: 7 days
Gender: male and female
Tissue: stria vascularis
Biomaterial provider Inna A. Belyantseva, NIDCD
Extracted molecule total RNA
 
Description Mouse vestibular tissue total RNA (MoVb.B16x) received from NIDCD was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Data processing Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
 
Submission date Jun 01, 2006
Last update date Nov 21, 2022
Contact name Robert Morell
E-mail(s) morellr@nidcd.nih.gov
Organization name National Institute on Deafness and Other Communication Disorders
Street address 35A Convent Drive, Room 1F-103
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL3835
Series (1)
GSE4965 Inner Ear Tissues

Data table header descriptions
ID_REF
VALUE Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
ST_DEV Standard deviation of the mean abundance from multiple MPSS runs
REP Replication_global

Data table
ID_REF VALUE ST_DEV REP
GATCGGTGTGACATCTGGTT 5 0 yes
GATCGGTCTCCAGAGTTTTA 23 0 yes
GATCGGTATTCGATGACGGT 8 8 yes
GATCGGTACTCGATGACGCT 10 9 yes
GATCGGGTTCAAATCTCTAC 5 0 yes
GATCCCAAGTTGAACAAAAG 3 2 yes
GATCCAGTCCCTGTCAAGCA 40 3 yes
GATCCCTACATCCCATAATC 13 2 yes
GATCATTCTGGGTCCAGTCA 2 2 yes
GATCCCATTCTATAGTGTGT 8 0 yes
GATCCGGCAGAAGGATGAGG 140 13 yes
GATCCGGATGTCCTAACTGA 7 2 yes
GATCCGGATGGACGCACAGC 10 10 yes
GATCCCATTAGACCTTGGGA 14 1 yes
GATCAAAAAGCGACGTCGCT 75 16 yes
GATCCCATTACAGGTGGTTG 27 1 yes
GATCATCTTCCTGTTGTAAA 4 4 yes
GATCCGGATGAAACATTGTC 173 5 yes
GATCCCATTAACAGCCCAGA 25 4 yes
GATCCGGACTCACCTGGGAG 78 18 yes

Total number of rows: 19743

Table truncated, full table size 572 Kbytes.




Supplementary data files not provided

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