|
| Status |
Public on Mar 25, 2016 |
| Title |
EBR_30 min_1 |
| Sample type |
RNA |
| |
|
| Source name |
Seedlings were cultured under exogenous 10 nM EBR treatments for 30 minutes
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: seedlings
treatment: 10 nM EBR
time: 30 min
|
| Treatment protocol |
Seedlings were then treated with medium containing 10 nM EBR or DMSO (mock) solution for 0.5 hours and 3 hours, respectively.
|
| Growth protocol |
Seedlings were grown in 1/2 MS medium with 1.5% sucrose. After stratification, seeds were transferred into 50 ml flasks with 10 ml liquid medium and incubated for seven days at 50 rpm and 22 °C under continuous light conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from complete frozen seedlings using TRIzol® Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's recommendations, and RNA purity was confirmed by spectrophotometry (A260/A280 ratio) and capillary electrophoresis (Agilent 2100 Bioanalyzer, Agilent Technologies, Palo Alto, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
100 ng total RNAs of each sample were prepared for labeling with Cyanine 3-pCp using the One-Color RNA Amplification kit (Agilent) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
RNA processing and hybridization were performed using miRBASE V14 arrays (Agilent Technologies, Palo Alto, CA, USA) according to the manufacturer’s protocol (this release contains 161 Arabidopsis thaliana miRNA genes).
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
Gene expression after 30 min in EBR-treated Arabidopsis thaliana
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
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| |
|
| Submission date |
Apr 25, 2013 |
| Last update date |
Mar 25, 2016 |
| Contact name |
Hsueh-Fen Juan |
| Organization name |
National Taiwan university
|
| Department |
institute of molecular and cellular biology
|
| Street address |
No. 1, Sec. 4, Roosevelt Road
|
| City |
Taipei |
| ZIP/Postal code |
10617 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL17065 |
| Series (1) |
| GSE46377 |
24-epibrassinolide regulates the expression of miRNAs in Arabidopsis thaliana |
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