|
Status |
Public on Apr 27, 2013 |
Title |
SS_A2_d3263.3 |
Sample type |
RNA |
|
|
Source name |
Rv3263 deletion mutant replicate 3
|
Organism |
Mycobacterium tuberculosis H37Rv |
Characteristics |
strain: H37Rv genotype: deltaRv3263
|
Growth protocol |
Cultures were grown to OD 0.8-0.9 in 7H9 supplemented with OADC, 0.05% Tween-80, and 0.2% glycerol in roller bottles
|
Extracted molecule |
total RNA |
Extraction protocol |
Cultures were inactivated with RNAlater, disrupted with bead-beating in the presence of Trizol, and total RNA was extracted according to the manufacturer's instructions. RNA was then treated with Dnase and re-purified with RNeasy.
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol for prokaryotic microarrays
|
|
|
Hybridization protocol |
Affymetrix protocol recommended for Pseudomonas aeruginosa
|
Scan protocol |
Microarrays were scanned using Affymetrix GeneArray Scanner 3000 7G Plus according to the manufacturer's protocol.
|
Description |
Gene expression data from an M. tuberculosis methyltransferase deletion mutant
|
Data processing |
Gene expression estimates were derived from the probe-level hybridization intensities using the RMA algorithm through the Expression Console software package (Affymetrix) using library files obtained from the array manufacturer (Affymetrix).
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|
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Submission date |
Apr 26, 2013 |
Last update date |
Apr 29, 2013 |
Contact name |
Scarlet Shell |
E-mail(s) |
sshell@wpi.edu
|
Organization name |
Worcester Polytechnic Institute
|
Department |
Biology and Biotechnology
|
Lab |
Scarlet Shell
|
Street address |
60 Prescott St
|
City |
Worcester |
State/province |
MA |
ZIP/Postal code |
01605 |
Country |
USA |
|
|
Platform ID |
GPL17082 |
Series (1) |
GSE46432 |
Analysis of the effects of the methyltransferase encoded by Rv3263 on gene expression in M. tuberculosis |
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