|
| Status |
Public on Apr 27, 2013 |
| Title |
SS_A3_Comp.3 |
| Sample type |
RNA |
| |
|
| Source name |
Complemented Rv3263 deletion mutant replicate 3
|
| Organism |
Mycobacterium tuberculosis H37Rv |
| Characteristics |
strain: H37Rv
genotype: deltaRv3263 complemented
|
| Growth protocol |
Cultures were grown to OD 0.8-0.9 in 7H9 supplemented with OADC, 0.05% Tween-80, and 0.2% glycerol in roller bottles
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cultures were inactivated with RNAlater, disrupted with bead-beating in the presence of Trizol, and total RNA was extracted according to the manufacturer's instructions. RNA was then treated with Dnase and re-purified with RNeasy.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol for prokaryotic microarrays
|
| |
|
| Hybridization protocol |
Affymetrix protocol recommended for Pseudomonas aeruginosa
|
| Scan protocol |
Microarrays were scanned using Affymetrix GeneArray Scanner 3000 7G Plus according to the manufacturer's protocol.
|
| Description |
Gene expression data from a complemented M. tuberculosis methyltransferase deletion mutant
|
| Data processing |
Gene expression estimates were derived from the probe-level hybridization intensities using the RMA algorithm through the Expression Console software package (Affymetrix) using library files obtained from the array manufacturer (Affymetrix).
|
| |
|
| Submission date |
Apr 26, 2013 |
| Last update date |
Apr 29, 2013 |
| Contact name |
Scarlet Shell |
| E-mail(s) |
sshell@wpi.edu
|
| Organization name |
Worcester Polytechnic Institute
|
| Department |
Biology and Biotechnology
|
| Lab |
Scarlet Shell
|
| Street address |
60 Prescott St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL17082 |
| Series (1) |
| GSE46432 |
Analysis of the effects of the methyltransferase encoded by Rv3263 on gene expression in M. tuberculosis |
|
