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Sample GSM1132544 Query DataSets for GSM1132544
Status Public on Sep 11, 2013
Title Virgin mouse_liver tissue_formulation-only_5d, biological rep 2
Sample type RNA
 
Source name SKH:QS mouse liver tissue from formulation-only-treated virgin mouse, day 5
Organism Mus musculus
Characteristics strain: SKH:QS
tissue: Virgin liver
Treatment protocol Mice were fitted with Elizabethan collars to minimise oral ingestion of sunscreens. Mice were treated six times over four days with sunscreen containing 68ZnO microparticles, 68ZnO nanoparticles, or no ZnO particles at all (formulation-only), or were untreated. Mice were sacrificed on day five by intraperitoneal injection of Xylase (50mg/kg)/ketamine (50mg/kg) followed by cervical dislocation.
Growth protocol Mice were individually housed in polycarbonate cages in an isolated temperature (~21˚C) and moisture (~55-65% relative humidity) controlled room with a 14-hr light/10-hr dark cycle, with ad libitum access to food and water
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from liver tissue using the NucleoSpin® RNA II kit (Macherey Nagel, Scientifix) following manufacturer’s instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip Whole Transcript (WT) Sense Target Labeling Assay User Manual, Affymetrix).
 
Hybridization protocol Following fragmentation, ~600-700 ng of single-stranded DNA were hybridised on Affymetric 1.0 ST human gene arrays (17 h, 45˚C, 60 rpm) in an Affymetrix 640 GeneChip® Hybridization Oven. GeneChips were washed and stained in an Affymetrix GeneChip® Fluidics Station 450.
Scan protocol GeneChips were scanned using an Affymetrix 7G GeneChip® Scanner.
Description Gene expression data from liver tissue harvested from virgin SKH:QS mouse topically treated with a sunscreen formulation-only six times over four days
Data processing The data was processed and analysed using Matlab Bioinformatics and Statistical toolboxes. RMA was used as the normalisation method. In-house tools were used to remove batch effects and control for false discovery.
 
Submission date May 02, 2013
Last update date Sep 11, 2013
Contact name Megan Osmond-McLeod
E-mail(s) megan.osmond@csiro.au
Organization name CSIRO
Street address 11 Julius Avenue
City North Ryde
ZIP/Postal code 1670
Country Australia
 
Platform ID GPL6246
Series (1)
GSE46568 Expression data from mouse liver tissue from SKH:QS mice treated with 68ZnO sunscreens

Data table header descriptions
ID_REF
VALUE log2 gene expression levels

Data table
ID_REF VALUE
10338001 12.347038
10338003 10.716095
10338004 9.6262646
10338017 13.058494
10338025 9.1553841
10338026 13.537772
10338029 9.7833595
10338035 9.304307
10338036 9.5884485
10338037 3.942713
10338041 11.417578
10338042 10.513257
10338044 12.497147
10338047 6.8049207
10338056 2.6823421
10338059 13.532835
10338060 4.070363
10338063 3.5648654
10338064 5.8281355
10338065 6.767982

Total number of rows: 35512

Table truncated, full table size 655 Kbytes.




Supplementary file Size Download File type/resource
GSM1132544_Not_Pregnant__Formulation_Only-2.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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