|
| Status |
Public on Oct 30, 2015 |
| Title |
HSY control, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
human parotid adenocarcinoma cell line, luciferase shRNA control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: fusion-negative HSY parotid adenocarcinoma cells
genotype/variataion: luciferase shRNA control
|
| Treatment protocol |
Cells were infected with retroviruses twice on two consecutive dates. Infected cells were subjected to FACS sorting for GFP+ cells 72 hours after 1st infection and total RNA were isolated.
|
| Growth protocol |
Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% inactivated fetal bovine serum at 37°C under 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the Trizol Reagent (Invitrogen) and purified by the Qiagen Rneasy Column with in column DNase digestion
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 plus 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner
|
| Description |
Gene expression data from fusion negative salivary gland tumor cells, control
|
| Data processing |
The data were normalized using MAS5 normalization approach with Affymetrix GCOS software.
|
| |
|
| Submission date |
May 02, 2013 |
| Last update date |
Jul 11, 2024 |
| Contact name |
Lizi Wu |
| Organization name |
University of Florida
|
| Street address |
2033 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE57022 |
Identification of MAML2 regulated transcriptional program in HSY cells |
| GSE59795 |
CRTC1-MAML2 fusion oncogene-induced transcriptional program is regulated by CREB-dependent and -independent mechanisms |
|
