|
| Status |
Public on Aug 02, 2013 |
| Title |
Day Time Treatment 1 |
| Sample type |
RNA |
| |
|
| Source name |
Aerial Rosete_Day Time Treatment 1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
developmental stage: seedling
tissue: aerial rosette
genotype/variation: WT
ecotype: Columbia
time: CT=18
treatment: 1hr white light pulse
|
| Treatment protocol |
1hr white light pulse during middle of the night (CT=6) or middle of the subjective day (CT=18). Control samples were collected without light pulse treatment.
|
| Growth protocol |
15 days old plant were grown in 12/12 white light/dark cycles and then were transfered to countinuos dark free running conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using RNaeasy Mini kit system according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Arabidopsis Genome ATH1 Array were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G
|
| Description |
Subj Day Rep 1
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 03, 2013 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Marcelo Yanovsky |
| E-mail(s) |
myanovsky@leloir.org.ar
|
| Organization name |
Fundación Instituto Leloir. IIBBA- CONICET
|
| Lab |
Laboratorio de Genómica Comparativa del Desarrollo Vegetal
|
| Street address |
Av. Patricias Argentinas 435
|
| City |
Buenos Aires |
| State/province |
Ciudad Autonoma de Buenos Aires |
| ZIP/Postal code |
C1405BWE |
| Country |
Argentina |
| |
|
| Platform ID |
GPL198 |
| Series (2) |
| GSE46621 |
Expression data from Arabidopsis thaliana seedlings |
| GSE46741 |
Arabidopsis circadian regulatory networks |
|
| Relations |
| Reanalyzed by |
GSE118579 |
