|
| Status |
Public on Mar 28, 2014 |
| Title |
hFib2-1 [genex] |
| Sample type |
RNA |
| |
|
| Source name |
Human normal Fibroblast replicate1
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: normal
individual: control
cell type: fibroblast
passage: control
|
| Growth protocol |
Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
|
| Label |
biotin
|
| Label protocol |
Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
|
| |
|
| Hybridization protocol |
cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
|
| Scan protocol |
Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
|
| Description |
Gene expression data obtained from Normal Human fibroblast
|
| Data processing |
RMA
|
| |
|
| Submission date |
May 11, 2013 |
| Last update date |
Mar 28, 2014 |
| Contact name |
Hu Li |
| E-mail(s) |
li.hu@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Molecular Pharmacology & Experimental Therapeutics
|
| Lab |
Systems Biology and Pharmacology
|
| Street address |
200 First Street, Gonda Building G19-408
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55904 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE46848 |
The distribution of genomic variations in human iPSCs is related to replication timing reorganization during reprogramming |
| GSE55395 |
The distribution of genomic variations in human iPSCs is related to replication timing reorganization during reprogramming [Affymetrix] |
|
