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Sample GSM1139124 Query DataSets for GSM1139124
Status Public on Mar 28, 2014
Title hFib2-2 [genex]
Sample type RNA
 
Source name Human normal Fibroblast replicate2
Organism Homo sapiens
Characteristics disease state: normal
individual: control
cell type: fibroblast
passage: control
Growth protocol Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
Extracted molecule total RNA
Extraction protocol Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
Label biotin
Label protocol Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
 
Hybridization protocol cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
Scan protocol Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
Description Gene expression data obtained from Normal Human fibroblast
Data processing RMA
 
Submission date May 11, 2013
Last update date Mar 28, 2014
Contact name Hu Li
E-mail(s) li.hu@mayo.edu
Organization name Mayo Clinic
Department Molecular Pharmacology & Experimental Therapeutics
Lab Systems Biology and Pharmacology
Street address 200 First Street, Gonda Building G19-408
City Rochester
State/province MN
ZIP/Postal code 55904
Country USA
 
Platform ID GPL570
Series (2)
GSE46848 The distribution of genomic variations in human iPSCs is related to replication timing reorganization during reprogramming
GSE55395 The distribution of genomic variations in human iPSCs is related to replication timing reorganization during reprogramming [Affymetrix]

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1007_s_at 9.243428
1053_at 8.252907
117_at 5.908313
121_at 8.625715
1255_g_at 4.685177
1294_at 7.726215
1316_at 8.080312
1320_at 6.237516
1405_i_at 3.872338
1431_at 4.673383
1438_at 7.22643
1487_at 8.613358
1494_f_at 6.11889
1552256_a_at 7.02352
1552257_a_at 8.871654
1552258_at 6.178492
1552261_at 5.383
1552263_at 8.194481
1552264_a_at 8.711059
1552266_at 3.840419

Total number of rows: 54613

Table truncated, full table size 1050 Kbytes.




Supplementary file Size Download File type/resource
GSM1139124_hFib2-2.CEL.gz 5.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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