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Sample GSM1143126 Query DataSets for GSM1143126
Status Public on Jan 01, 2016
Title SCC13 control cells, 13_EV_1
Sample type RNA
 
Source name SCC13 human cell line, control
Organism Homo sapiens
Characteristics cell line: SCC13
stable infection: scrambled control hairpin
batch infection: 1
technical replicate: 1
Treatment protocol Total RNA (including small RNAs) was isolated from cultured cells stably infected in biological duplicate with either a scrambled control hairpin or miR-203. Samples were harvested in technical duplicate.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a miRNeasy Mini Kit (Qiagen) and RNA quality was analyzed using a Bioanalyzer 2100 (Agilent) and RNA Nano Chip. RIN values were all above 9.3.
Label Biotin
Label protocol Standard Illumina labeling protocol.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description Established squamous cell carcinoma cell line from facial SCC.
Data processing The data was log base 2 quantile normalised after artifact removal using the BASH and HULK algorithms in the R beadarray package.
 
Submission date May 16, 2013
Last update date Jan 01, 2016
Contact name Nathan Benaich
Organization name King's College London
Department Centre for Stem Cells and Regenerative Medicine
Lab 28th Floor, Tower Wing, Guy's Hospital
Street address Great Maze Pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
 
Platform ID GPL10558
Series (1)
GSE47028 miR-203, a microRNA of multilayered epithelia, inhibits metastatic fitness in human squamous cell carcinoma

Data table header descriptions
ID_REF
VALUE Log2 quantile-normalised signal intensity

Data table
ID_REF VALUE
ILMN_1802380 8.097879682
ILMN_1893287 7.096595714
ILMN_3238331 6.925878742
ILMN_1736104 7.099918338
ILMN_1792389 7.391646415
ILMN_1854015 7.736108153
ILMN_3308818 7.193808493
ILMN_1740305 7.512525265
ILMN_3242405 7.431553331
ILMN_1665168 7.238727484
ILMN_2375156 7.488490618
ILMN_1705423 7.29475584
ILMN_1716072 6.978469406
ILMN_1697642 10.09065659
ILMN_3295558 7.064651359
ILMN_1788184 7.203477866
ILMN_1681845 9.2769784
ILMN_3228430 7.086611035
ILMN_1746923 7.281740082
ILMN_1690979 7.209898001

Total number of rows: 47231

Table truncated, full table size 1147 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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