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Sample GSM115445 Query DataSets for GSM115445
Status Public on Oct 01, 2006
Title FRTL5-17hrs-rep1
Sample type RNA
 
Source name FRTL5 cells, 17 hrs TSH-stimulated
Organism Rattus norvegicus
Characteristics Fisher Rat Thyroid Cell line (FRT/TSHR), early passage
Treatment protocol Cells were stimulated with 0.1 U/L bovine TSH
Growth protocol Cells were grown in TSH-free medium (5H) for 5 days. Twelve hours before bTSH stimulation, cells were starved in serum-free medium
Extracted molecule total RNA
Extraction protocol total RNA samples were isolated using Trizol reagent (Invitrogen) and purified on RNeasy spin columns (Quiagen). Double-stranded cDNA was synthesized from 10 mcg RNA using Superscript Double Stranded cDNA synthesis kit (Invitrogen), phenol/chloroform extracted and ethanol precipitated.
Label biotin
Label protocol Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets, using the BioArray high yield RNA transcript labeling kit (Enzo Diagnostic).
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description differentiated rat thyrocytes stimulated with TSH
Data processing data were quantile normalized and processed using the Robust Microarray Analysis (RMA) algorithm, available at http://www.bioconductor.org, under R statistical environment (Irizarry et al, 2003).
 
Submission date Jun 21, 2006
Last update date Jun 21, 2006
Contact name Davide Calebiro
E-mail(s) davide.calebiro@guest.unimi.it
Organization name Istituto Auxologico Italiano
Lab Lab. of Exp. Endocrinology
Street address via Zucchi 18
City Cusano Milanino
ZIP/Postal code 20095
Country Italy
 
Platform ID GPL85
Series (1)
GSE5115 Time course analysis of TSH-stimulated rat thyroid cell lines

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
A01157cds_s_at 4.653549186
A03913cds_s_at 4.123942917
A04674cds_s_at 4.176488452
A07543cds_s_at 5.017233091
A09811cds_s_at 5.357279784
A16585cds_s_at 4.891988705
A17753cds_s_at 4.771982905
A30543cds_s_at 4.486252305
A44407cds_at 5.572009649
AA108277_at 4.145508415
AA108308_i_at 3.43494915
AA108308_s_at 7.021125446
AA684537_at 7.694224196
AA684929_f_at 6.259210376
AA684960_f_at 6.081049262
AA684963_at 7.464727705
AA685112_at 7.371989438
AA685152_r_at 6.039813885
AA685376_f_at 5.634252146
AA685876_at 7.497816833

Total number of rows: 8799

Table truncated, full table size 216 Kbytes.




Supplementary data files not provided

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