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Sample GSM115447 Query DataSets for GSM115447
Status Public on Oct 01, 2006
Title FRTL5-17hrs-rep3
Sample type RNA
 
Source name FRTL5 cells, 17 hrs TSH-stimulated
Organism Rattus norvegicus
Characteristics Fisher Rat Thyroid Cell line (FRT/TSHR), early passage
Treatment protocol Cells were stimulated with 0.1 U/L bovine TSH
Growth protocol Cells were grown in TSH-free medium (5H) for 5 days. Twelve hours before bTSH stimulation, cells were starved in serum-free medium
Extracted molecule total RNA
Extraction protocol total RNA samples were isolated using Trizol reagent (Invitrogen) and purified on RNeasy spin columns (Quiagen). Double-stranded cDNA was synthesized from 10 mcg RNA using Superscript Double Stranded cDNA synthesis kit (Invitrogen), phenol/chloroform extracted and ethanol precipitated.
Label biotin
Label protocol Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets, using the BioArray high yield RNA transcript labeling kit (Enzo Diagnostic).
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description differentiated rat thyrocytes stimulated with TSH
Data processing data were quantile normalized and processed using the Robust Microarray Analysis (RMA) algorithm, available at http://www.bioconductor.org, under R statistical environment (Irizarry et al, 2003).
 
Submission date Jun 21, 2006
Last update date Jun 21, 2006
Contact name Davide Calebiro
E-mail(s) davide.calebiro@guest.unimi.it
Organization name Istituto Auxologico Italiano
Lab Lab. of Exp. Endocrinology
Street address via Zucchi 18
City Cusano Milanino
ZIP/Postal code 20095
Country Italy
 
Platform ID GPL85
Series (1)
GSE5115 Time course analysis of TSH-stimulated rat thyroid cell lines

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
A01157cds_s_at 4.848211468
A03913cds_s_at 4.024783447
A04674cds_s_at 4.46275367
A07543cds_s_at 4.729170233
A09811cds_s_at 5.545160303
A16585cds_s_at 4.778051604
A17753cds_s_at 4.842544345
A30543cds_s_at 5.065567338
A44407cds_at 6.667546791
AA108277_at 5.090487215
AA108308_i_at 4.218433457
AA108308_s_at 7.801139945
AA684537_at 8.486802469
AA684929_f_at 6.467028293
AA684960_f_at 6.305563584
AA684963_at 8.461810534
AA685112_at 7.742530731
AA685152_r_at 6.799039683
AA685376_f_at 6.129296805
AA685876_at 9.02669952

Total number of rows: 8799

Table truncated, full table size 216 Kbytes.




Supplementary data files not provided

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