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Sample GSM1172683 Query DataSets for GSM1172683
Status Public on Jul 22, 2013
Title CiPS45
Sample type RNA
Source name Mouse embryonic fibroblasts
Organism Mus musculus
Characteristics development stage: embryo
cell type: pluripotent stem cell
cell line: CiPS line 45
chemical treatment conditon: FC6+Z
Growth protocol MEFs, MNFs, MAFs and ADSCs were cultured in DMEM/High Glucose (Hyclone) containing 10% fetal bovine serum (Invitrogen). Mouse ESCs (R1 and TT2), iPSCs and CiPSCs were maintained on feeder layers of mitomycin C-treated MEFs in ESC culture medium.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol and purified by QIAGEN Rneasy Kit following manufacturer's instructions.
Label Cy5
Label protocol Total mRNAs of all samples were labeled with Cy5 following the Mouse Onearray User Manual V2 (Phalanx Biotech Group).
Hybridization protocol Hybridized to a mouse Oligo Microarray following the Mouse Onearray User Manual V2 (Phalanx Biotech Group).
Scan protocol Data extracton used GenePix 4100B (Molecular Devices) scanner from molecular Devices, and measured using GenePixPro Software (Molecular Devices) .
Description Mouse Whole Genome OneArray® v2 (Phalanx Biotech Group, Taiwan)
Data processing Primary data selected by Flags and SNRs. After selection, average or median value of each sample were normalized using matlab.
Submission date Jun 21, 2013
Last update date Jul 22, 2013
Contact name chun liu
Phone 13621043040
Organization name Peking University
Street address beijing
City beijing
State/province beijing
ZIP/Postal code 100871
Country China
Platform ID GPL15523
Series (2)
GSE48201 Inducing pluripotency from mouse somatic cells by small-molecule compounds [Phalanx Mouse OneArray]
GSE48243 Inducing pluripotency from mouse somatic cells by small-molecule compounds

Data table header descriptions
VALUE Data were analyzed according to the manufacturer's protocol (Phalanx Mouse OneArray® v2, Phalanx Biotech) after removing control probes.

Data table
mMC000772 427.6
mMC000773 60.6
mMC000774 308.3
mMC000780 158.9
mMC000784 41.5
mMC000785 65.4
mMC000790 80.5
mMC000796 2623.5
mMC000801 1003
mMC000804 232
mMC000811 637.7
mMC000813 648.6

Total number of rows: 26423

Table truncated, full table size 429 Kbytes.

Supplementary file Size Download File type/resource
GSM1172683_CiPS45-1.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM1172683_CiPS45-2.gpr.gz 2.7 Mb (ftp)(http) GPR
GSM1172683_CiPS45-3.gpr.gz 2.7 Mb (ftp)(http) GPR
Processed data included within Sample table

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