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Sample GSM1172689 Query DataSets for GSM1172689
Status Public on Jul 22, 2013
Title CiPS WT2
Sample type RNA
 
Source name Wildtyp mouse embryonic fibroblasts
Organism Mus musculus
Characteristics development stage: embryo
cell type: pluripotent stem cell
cell line: CiPS WT line 2
chemical treatment conditon: VC6TFMPS+Z
Growth protocol MEFs, MNFs, MAFs and ADSCs were cultured in DMEM/High Glucose (Hyclone) containing 10% fetal bovine serum (Invitrogen). Mouse ESCs (R1 and TT2), iPSCs and CiPSCs were maintained on feeder layers of mitomycin C-treated MEFs in ESC culture medium.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol and purified by QIAGEN Rneasy Kit following manufacturer's instructions.
Label Cy5
Label protocol Total mRNAs of all samples were labeled with Cy5 following the Mouse Onearray User Manual V2 (Phalanx Biotech Group).
 
Hybridization protocol Hybridized to a mouse Oligo Microarray following the Mouse Onearray User Manual V2 (Phalanx Biotech Group).
Scan protocol Data extracton used GenePix 4100B (Molecular Devices) scanner from molecular Devices, and measured using GenePixPro Software (Molecular Devices) .
Description Mouse Whole Genome OneArray® v2 (Phalanx Biotech Group, Taiwan)
Data processing Primary data selected by Flags and SNRs. After selection, average or median value of each sample were normalized using matlab.
 
Submission date Jun 21, 2013
Last update date Jul 22, 2013
Contact name chun liu
E-mail(s) dabai22222@163.com
Phone 13621043040
Organization name Peking University
Street address beijing
City beijing
State/province beijing
ZIP/Postal code 100871
Country China
 
Platform ID GPL15523
Series (2)
GSE48201 Inducing pluripotency from mouse somatic cells by small-molecule compounds [Phalanx Mouse OneArray]
GSE48243 Inducing pluripotency from mouse somatic cells by small-molecule compounds

Data table header descriptions
ID_REF
VALUE Data were analyzed according to the manufacturer's protocol (Phalanx Mouse OneArray® v2, Phalanx Biotech) after removing control probes.

Data table
ID_REF VALUE
mMC000770 10861.125
mMC000772 506
mMC000773 73.5
mMC000774 336.75
mMC000776
mMC000780 115.25
mMC000784 27
mMC000785 55.375
mMC000790 63.625
mMC000792
mMC000796 2132.125
mMC000801 2030.5
mMC000804 244.375
mMC000805
mMC000811 609.625
mMC000813 167.5
mMC000817 1200.875
mMC000821
mMC000826
mMC000828

Total number of rows: 26423

Table truncated, full table size 450 Kbytes.




Supplementary file Size Download File type/resource
GSM1172689_CiPS_WT2-1.gpr.gz 2.6 Mb (ftp)(http) GPR
GSM1172689_CiPS_WT2-2.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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