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Sample GSM1172690 Query DataSets for GSM1172690
Status Public on Jul 22, 2013
Title Embryonic stem cells3
Sample type RNA
Source name Embryonic stem cell line R1
Organism Mus musculus
Characteristics development stage: embryo
cell type: Embryonic stem cell
cell line: Embryonic stem cell line 3
chemical treatment conditon: no treatment
Growth protocol MEFs, MNFs, MAFs and ADSCs were cultured in DMEM/High Glucose (Hyclone) containing 10% fetal bovine serum (Invitrogen). Mouse ESCs (R1 and TT2), iPSCs and CiPSCs were maintained on feeder layers of mitomycin C-treated MEFs in ESC culture medium.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol and purified by QIAGEN Rneasy Kit following manufacturer's instructions.
Label Cy5
Label protocol Total mRNAs of all samples were labeled with Cy5 following the Mouse Onearray User Manual V2 (Phalanx Biotech Group).
Hybridization protocol Hybridized to a mouse Oligo Microarray following the Mouse Onearray User Manual V2 (Phalanx Biotech Group).
Scan protocol Data extracton used GenePix 4100B (Molecular Devices) scanner from molecular Devices, and measured using GenePixPro Software (Molecular Devices) .
Description Mouse Whole Genome OneArray® v2 (Phalanx Biotech Group, Taiwan)
Data processing Primary data selected by Flags and SNRs. After selection, average or median value of each sample were normalized using matlab.
Submission date Jun 21, 2013
Last update date Jul 22, 2013
Contact name chun liu
Phone 13621043040
Organization name Peking University
Street address beijing
City beijing
State/province beijing
ZIP/Postal code 100871
Country China
Platform ID GPL15523
Series (2)
GSE48201 Inducing pluripotency from mouse somatic cells by small-molecule compounds [Phalanx Mouse OneArray]
GSE48243 Inducing pluripotency from mouse somatic cells by small-molecule compounds

Data table header descriptions
VALUE Data were analyzed according to the manufacturer's protocol (Phalanx Mouse OneArray® v2, Phalanx Biotech) after removing control probes.

Data table
mMC000770 16293.875
mMC000772 695.375
mMC000773 74.375
mMC000774 375.75
mMC000780 91.25
mMC000784 24.75
mMC000785 53.625
mMC000790 142.875
mMC000796 1959.8125
mMC000801 1786.25
mMC000804 88
mMC000811 621.4375
mMC000813 169
mMC000817 893.5

Total number of rows: 26423

Table truncated, full table size 450 Kbytes.

Supplementary file Size Download File type/resource
GSM1172690_ESCs3-1.gpr.gz 2.7 Mb (ftp)(http) GPR
GSM1172690_ESCs3-2.gpr.gz 2.7 Mb (ftp)(http) GPR
Processed data included within Sample table

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