Total RNA was extracted using RNeasy Mini(QIAGEN).
Label
Cy3
Label protocol
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008).
Hybridization protocol
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008).
Scan protocol
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008).
Description
Tissue
Data processing
The scanned images were analyzed with Feature Extraction Software 9.5 (Agilent) using default parameters (protocol GE1_v5_95_Feb07 and Grid: 014879_D_20070207) to obtain background subtracted and spatially detrended Processed Signal intensities.
Transcriptome analysis of mesothelioma induced by intraperitoneal injections of asbestos in rats
Data table header descriptions
ID_REF
VALUE
Signal intensity after normalization by GeneSpring GX11.5 software (Agilent). [Threshold: 1.0, Logbase: 2, Normalization: Shift to 75 percentile, Baseline Transformation: Median of Control Samples[mes_tissue1 and 2]]
