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Sample GSM1179059 Query DataSets for GSM1179059
Status Public on Jul 01, 2016
Title 003780_003780_63deltaMeq(96)
Sample type RNA
 
Source name 003780_003780_63deltaMeq(96)
Organism Gallus gallus
Characteristics cell type: chicken embryo fibroblast cell line
infection: Meq-null MDV
time (h): 96
Growth protocol Cells were cultured in Lebowitz's L-15 and McCoy 5A media with 15% inactivated fetal bovine serum, 100 U of penicillin per ml, and maintained at 37 °C.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Absolutely RNA Miniprep Kit (Stratagene, Santa Clara, CA)
Label biotin
Label protocol High quality RNA was labeled using Affymetrix (Santa Clara, CA) One-Cycle Target Labeling and Control Reagents
 
Hybridization protocol Biotinylated cRNA was hybridized to the Affymetrix chips (GeneChip Chicken Genome Array). GeneChips were then washed and stained in the Affymetrix Fluidics Station
Scan protocol Genechips were scanned and the resultant intensities analyzed for levels of transcripts of interest
Data processing The data were analyzed with Affymetrix using default analysis settings and global scaling as normalization method.
 
Submission date Jul 01, 2013
Last update date Jul 01, 2016
Contact name sugalesini Subramaniam
E-mail(s) subram28@msu.edu
Phone 5172302659
Organization name Michigan State University
Department CMIB
Lab USDA-ADOL
Street address 3606 E Mount Hope Rd
City East Lansing
State/province MI
ZIP/Postal code 48823
Country USA
 
Platform ID GPL3213
Series (2)
GSE48453 Transcriptional Profiling of Meq-dependent Genes in Marek’s Disease Resistant and Susceptible Inbred Chicken Lines
GSE48454 Genes and pathways involved in cellular transformation by Marek's disease oncoprotein Meq

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 9.18035
AFFX-BioB-M_at 9.72729
AFFX-BioB-3_at 9.19395
AFFX-BioC-5_at 10.1922
AFFX-BioC-3_at 9.48249
AFFX-BioDn-5_at 10.5344
AFFX-BioDn-3_at 12.0908
AFFX-CreX-5_at 13.2907
AFFX-CreX-3_at 13.4025
AFFX-DapX-5_at 6.19314
AFFX-DapX-M_at 7.47795
AFFX-DapX-3_at 8.15074
AFFX-LysX-5_at 3.30673
AFFX-LysX-M_at 3.83449
AFFX-LysX-3_at 5.27518
AFFX-PheX-5_at 4.04014
AFFX-PheX-M_at 4.24894
AFFX-PheX-3_at 6.29971
AFFX-ThrX-5_at 5.45204
AFFX-ThrX-M_at 5.32371

Total number of rows: 38535

Table truncated, full table size 1040 Kbytes.




Supplementary file Size Download File type/resource
GSM1179059_003780_003780_63deltaMeq_96_.CEL.gz 3.1 Mb (ftp)(http) CEL
GSM1179059_003780_003780_63deltaMeq_96_.rma.chp.gz 328.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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