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Sample GSM1179390 Query DataSets for GSM1179390
Status Public on Aug 30, 2013
Title 300nM_Topotecan_1
Sample type RNA
Source name cultured cortical neurons
Organism Mus musculus
Characteristics treatment: 300nM_Topotecan
cell type: cultured cortical neurons
strain: C57BL6
Treatment protocol After 7 days in vitro, mouse cortical neurons were treated with drug or vehicle. After 24 or 72 hrs drug treatement, total RNA was prepared.
Growth protocol Mouse cortical neurons were cultured from E13-15 mouse embryos for 7 days in vitro.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse cortical neurons using Trizol reagent.
Label biotin
Label protocol RNA was labeled using the Affymetrix/Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit.
Hybridization protocol Arrays for experiments (1) and (3) were washed and stained using the Affymetrix Gene Fluidics station 450. Hybridization was performed at 48˚C for 17 hours. Arrays for other experiments were run using the Affymetrix GeneTitan system. Steps subsequent to hybridization were performed with the Beckman Coulter FXP Target Express Robot. Hybridization was performed at 45˚C for 16 hours with the Affymetrix GeneTitan instrument, controlled using AGCC software.
Scan protocol GeneTitan arrays were scanned using the Affymetrix GeneTitan instrument, controlled by AGCC software. Individual arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with autoloader.
Description MoGene-1_1-st-v1
Gene Expression data for Topotecan treated mouse cultured cortical neurons
Data processing Arrays were normalized and expression values determined using Affymetrix Expression Console. Linear RMA normalization was used for all experiments.
Submission date Jul 02, 2013
Last update date Aug 30, 2013
Contact name Chandri Yandava
Organization name University of North Carolina at Chapel Hill
Department Carolina Institute for Developmental Disabilities
Street address 101 Renee Lynne Court
City Carrboro
State/province NC
ZIP/Postal code 27510
Country USA
Platform ID GPL11533
Series (2)
GSE43899 Co-ordinate inhibiton of autism candidate genes by topoisomerase inhibitors [array]
GSE43900 Co-ordinate inhibition of autism candidate genes by topoisomerase inhibitors

Data table header descriptions
VALUE Linear RMA signal

Data table
10344614 143.008
10344616 5.82344
10344620 11.94821
10344622 35.79726
10344624 540.767
10344633 639.6071
10344637 1383.15
10344653 13.46598
10344658 484.1738
10344674 12.38778
10344679 49.6804
10344707 943.1528
10344713 659.3635
10344715 24.25683
10344717 8.350092
10344719 24.2914
10344721 5.509747
10344723 121.0575
10344725 111.2185
10344741 982.3427

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.

Supplementary file Size Download File type/resource
GSM1179390_300nM_Topotecan_1.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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